X5J8S6 · X5J8S6_9BACT
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids368 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-17 | ATP (UniProtKB | ChEBI) | ||||
Sequence: AMSGGIDS | ||||||
Binding site | 36 | ATP (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Active site | 106 | Nucleophile | ||||
Sequence: C | ||||||
Binding site | 130 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Site | 131 | Interaction with tRNA | ||||
Sequence: H | ||||||
Active site | 203 | Cysteine persulfide intermediate | ||||
Sequence: C | ||||||
Site | 345 | Interaction with tRNA | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Cytophagia > Cytophagales > Amoebophilaceae > Candidatus Cardinium
Accessions
- Primary accessionX5J8S6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Disulfide bond | 106↔203 | Alternate | ||||
Sequence: CNTHIKWDALLQRANKLNCDYLATGHYAKVRPSGGRYIISKGLDGKKDQSYALWGVSQESLSRTLLPLGGYTKSFIRAFAAERGFTDLVHKSESYEIC |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 152-154 | Interaction with tRNA | ||||
Sequence: KDQ | ||||||
Domain | 226-278 | tRNA-specific 2-thiouridylase MnmA-like central | ||||
Sequence: KGGTFILEDGTVVGHHEGYPFYTIGQRKGLNIALGYPVYVTAIRKETNQVVLG | ||||||
Domain | 286-361 | tRNA-specific 2-thiouridylase MnmA-like C-terminal | ||||
Sequence: DGMYVHQLNLSKYIDLKGKKLDTLTKVRYNDPGTPAVIEQVGNQMHVFFGTGVHAVTPGQAAVFYEEEDVIGGGWI | ||||||
Region | 313-314 | Interaction with tRNA | ||||
Sequence: RY |
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length368
- Mass (Da)40,835
- Last updated2014-06-11 v1
- ChecksumB9BF9E7D1F4EECF4