X5J815 · X5J815_9BACT

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site21dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site51(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site126-127dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site146
Active site146Nucleophile
Binding site166-169dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site169(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site177dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site207-209dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site263(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • ORF names
      CHV_f0003

Organism names

Accessions

  • Primary accession
    X5J815

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain3-264Thymidylate synthase/dCMP hydroxymethylase

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    264
  • Mass (Da)
    30,270
  • Last updated
    2014-06-11 v1
  • Checksum
    4D2662D9028E62AE
MKPYLTLLQHILAEGVDKKDRTGTGTKSLFGYQMRFNLEEGFPLITTKKVHLRSIIYELLWFLSGDTNVRYLQEHGVTIWDEWASKEGRLGPIYGHQWRSWSTETGVKIDQISQTIKEIKTNPDSRRLLVSAWNVGEISKMALPPCHAFFQFYVANGKLSCQLYQRSADVFLGVPFNIASYGLLTMMMAQVCQLKPGTLIHTLGDAHLYYNHLEQASLQLKRSPYPLPTMQLNQNVTDLFSFVYEDFTLLHYLHHPAIKAVVSV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CBQZ010000006
EMBL· GenBank· DDBJ
CDG50113.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp