X5CPA9 · X5CPA9_9RHAB
- ProteinReplicase
- GeneL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids2128 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
RNA-directed RNA polymerase that catalyzes the replication of viral genomic RNA. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing transcriptional signals, and the replicated genome is not caped or polyadenylated.
Catalytic activity
- GTP + H2O = GDP + phosphate + H+
- a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
- a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine + H+
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | host cell cytoplasm | |
Cellular Component | virion component | |
Molecular Function | ATP binding | |
Molecular Function | hydrolase activity | |
Molecular Function | mRNA 5'-cap (guanine-N7-)-methyltransferase activity | |
Molecular Function | RNA-dependent RNA polymerase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Rhabdoviridae > Alpharhabdovirinae > Lyssavirus
Accessions
- Primary accessionX5CPA9
Proteomes
Subcellular Location
Interaction
Subunit
May form homodimer. Interacts with the P protein.
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-31 | Disordered | ||||
Sequence: MMIDPGEVYDDPVDPIESEAEPRGTPTVPNI | ||||||
Domain | 612-800 | RdRp catalytic | ||||
Sequence: VTYAFHLDYEKWNNHQRLESTEDVFSVLDQVFGLKRVFSRTHEFFQKSWIYYSDRSDLIGLWEDQIYCLDMSDGPTCWNGQDGGLEGLRQKGWSPVSLLMIDRESQTRNTRTKILAQGDNQVLCPTYMLSPGLSREGLLYELESISRNALSIYRAIEEGAAKLGLIIKKEETMCSYDFLIYGKTPLFRG | ||||||
Domain | 1675-1872 | Mononegavirus-type SAM-dependent 2'-O-MTase | ||||
Sequence: RVVQWATGAHYKLKPILDDLNVFPSLCLVVGDGSGGISRAVLNMFPDAKLVFNSLLEVNDLMASGTHPLPPSAIMSGGDEIVSRVIDFDSIWEKPSDLRNLTTWKYFQSVQKKVNMSYDLIICDAEVTDIASINRITLLMSDFALSIDGPLYLVLKTYGTMLVNPEYRAIQHLSRAFPSVTGFITQMTSSFSSELYLR |
Family and domain databases
Sequence
- Sequence statusComplete
- Length2,128
- Mass (Da)242,700
- Last updated2014-06-11 v1
- ChecksumD270A7D654C72B0F