X2FL49 · X2FL49_CHV16
- ProteinDNA polymerase
- GeneUL30
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1241 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Replicates viral genomic DNA. The replication complex is composed of six viral proteins: the DNA polymerase, processivity factor, primase, primase-associated factor, helicase, and ssDNA-binding protein. Additionally, the polymerase contains an intrinsic ribonuclease H (RNase H) activity that specifically degrades RNA/DNA heteroduplexes or duplex DNA substrates in the 5' to 3' direction. Therefore, it can catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during viral DNA replication.
Catalytic activity
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | DNA-templated DNA replication | |
Biological Process | viral DNA genome replication |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Alphaherpesvirinae > Simplexvirus > Simplexvirus papiinealpha2
- Virus hosts
Accessions
- Primary accessionX2FL49
Proteomes
Subcellular Location
Interaction
Subunit
Forms a complex with the ssDNA-binding protein UL29, the DNA polymerase processivity factor, and the alkaline exonuclease. Interacts with the putative helicase-primase complex subunit UL8; this interaction may coordinate leading and lagging strand DNA synthesis at the replication fork.
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-41 | Disordered | |||
Domain | 291-543 | DNA-directed DNA polymerase family B exonuclease | |||
Domain | 607-1181 | DNA-directed DNA polymerase family B multifunctional | |||
Region | 646-699 | Disordered | |||
Region | 1105-1138 | Disordered | |||
Compositional bias | 1107-1129 | Pro residues | |||
Domain | 1209-1241 | DNA polymerase catalytic subunit Pol C-terminal | |||
Sequence similarities
Belongs to the DNA polymerase type-B family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,241
- Mass (Da)135,859
- Last updated2014-06-11 v1
- MD5 Checksum4255064B8BDE3FEC414B9F96A8E20243
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1107-1129 | Pro residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KF908242 EMBL· GenBank· DDBJ | AHM96225.1 EMBL· GenBank· DDBJ | Genomic DNA |