W8U685 · W8U685_STAAU

  • Protein
    Protein/nucleic acid deglycase HchA
  • Gene
    hchA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
  • H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
  • H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
  • H2O + N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H+ + L-lysyl-[protein]
  • H2O + N6-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] = H+ + L-lysyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]
  • H2O + N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H+ + L-arginyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]
  • H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H+ + L-cysteinyl-[protein] + lactate
    EC:3.5.1.124 (UniProtKB | ENZYME | Rhea)
  • H2O + methylglyoxal = (R)-lactate + H+
    EC:4.2.1.130 (UniProtKB | ENZYME | Rhea)
  • an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
  • an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
  • an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site190Nucleophile

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionlyase activity
Molecular Functionprotein deglycase activity
Molecular Functionthiolester hydrolase activity
Biological ProcessDNA repair
Biological Processprotein repair

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Protein/nucleic acid deglycase HchA
  • EC number
  • Alternative names
    • Maillard deglycase

Gene names

    • Name
      hchA
    • ORF names
      AC807_1256
      , C0100_008100
      , CFN12_02695
      , DQV00_10705
      , EIG96_01590
      , EP54_12995
      , EQ90_07165
      , ERS092965_02696
      , ERS1058648_00553
      , ERS195417_02214
      , G0Z31_06350
      , G6W84_06890
      , G6Y24_14940
      , GO814_00260
      , GO942_12720
      , GQX37_06475
      , GQX52_08750
      , HT539_04560
      , M1K003_2012
      , NCTC13131_01033
      , SAMEA1029519_00276
      , SAMEA1965892_02286
      , SAMEA2078260_02178
      , SAMEA2078588_02177
      , SAMEA2079512_02324
      , SAMEA2080344_02232
      , SAMEA2080909_02366
      , SAMEA2081063_02170
      , SAMEA2081779_02152
      , SAMEA4008452_00552
      , SAMEA4008575_00555
      , SAMEA4552975_01275
      , SAMEA70146418_00935
      , SAMEA70153168_02265

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • USA300_2014.C01
    • CA15
    • M121
    • FL383
    • USFL204
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus

Accessions

  • Primary accession
    W8U685
  • Secondary accessions
    • A0A1E8X2U6

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-23Disordered
Domain79-204DJ-1/PfpI

Sequence similarities

Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    292
  • Mass (Da)
    32,177
  • Last updated
    2014-05-14 v1
  • Checksum
    7ADA2635287373CD
MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTAPKTDFDGVEHKGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLSGYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPDSLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNELGKLAVNEMLNAIQNK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP012119
EMBL· GenBank· DDBJ
AKR51333.1
EMBL· GenBank· DDBJ
Genomic DNA
CP022290
EMBL· GenBank· DDBJ
ATW76519.1
EMBL· GenBank· DDBJ
Genomic DNA
CACTPC010000001
EMBL· GenBank· DDBJ
CAA4077511.1
EMBL· GenBank· DDBJ
Genomic DNA
CACTQT010000013
EMBL· GenBank· DDBJ
CAA4389431.1
EMBL· GenBank· DDBJ
Genomic DNA
CACTSG010000014
EMBL· GenBank· DDBJ
CAA4449620.1
EMBL· GenBank· DDBJ
Genomic DNA
CACTWD010000014
EMBL· GenBank· DDBJ
CAA4697153.1
EMBL· GenBank· DDBJ
Genomic DNA
CACTWS010000012
EMBL· GenBank· DDBJ
CAA4776311.1
EMBL· GenBank· DDBJ
Genomic DNA
CACUKU010000012
EMBL· GenBank· DDBJ
CAA5874430.1
EMBL· GenBank· DDBJ
Genomic DNA
CACUNS010000014
EMBL· GenBank· DDBJ
CAA6110482.1
EMBL· GenBank· DDBJ
Genomic DNA
CACUOU010000016
EMBL· GenBank· DDBJ
CAA6139993.1
EMBL· GenBank· DDBJ
Genomic DNA
CACURZ010000014
EMBL· GenBank· DDBJ
CAA6376152.1
EMBL· GenBank· DDBJ
Genomic DNA
CAIGSM010000001
EMBL· GenBank· DDBJ
CAC5478115.1
EMBL· GenBank· DDBJ
Genomic DNA
CAIGXB010000001
EMBL· GenBank· DDBJ
CAC5778289.1
EMBL· GenBank· DDBJ
Genomic DNA
CAIHOM010000001
EMBL· GenBank· DDBJ
CAC6998376.1
EMBL· GenBank· DDBJ
Genomic DNA
CAIIGD010000002
EMBL· GenBank· DDBJ
CAC8204304.1
EMBL· GenBank· DDBJ
Genomic DNA
CAIIGN010000009
EMBL· GenBank· DDBJ
CAC8249767.1
EMBL· GenBank· DDBJ
Genomic DNA
UAUZ02000002
EMBL· GenBank· DDBJ
CAD7353547.1
EMBL· GenBank· DDBJ
Genomic DNA
CSGC01000010
EMBL· GenBank· DDBJ
CPC09472.1
EMBL· GenBank· DDBJ
Genomic DNA
CSIU01000013
EMBL· GenBank· DDBJ
CPE21495.1
EMBL· GenBank· DDBJ
Genomic DNA
FJMW01000001
EMBL· GenBank· DDBJ
CZQ26515.1
EMBL· GenBank· DDBJ
Genomic DNA
BDVT01000010
EMBL· GenBank· DDBJ
GBV21010.1
EMBL· GenBank· DDBJ
Genomic DNA
LALJ01000012
EMBL· GenBank· DDBJ
KMR36711.1
EMBL· GenBank· DDBJ
Genomic DNA
LALQ01000066
EMBL· GenBank· DDBJ
KMR56028.1
EMBL· GenBank· DDBJ
Genomic DNA
JABXAU010000001
EMBL· GenBank· DDBJ
MBB2557044.1
EMBL· GenBank· DDBJ
Genomic DNA
PKSS02000011
EMBL· GenBank· DDBJ
MBW7464080.1
EMBL· GenBank· DDBJ
Genomic DNA
WPTS01000002
EMBL· GenBank· DDBJ
MVK33584.1
EMBL· GenBank· DDBJ
Genomic DNA
WPXC01000032
EMBL· GenBank· DDBJ
MVM11526.1
EMBL· GenBank· DDBJ
Genomic DNA
JAAJIY010000015
EMBL· GenBank· DDBJ
NGK21137.1
EMBL· GenBank· DDBJ
Genomic DNA
JAALWO010000012
EMBL· GenBank· DDBJ
NGV46844.1
EMBL· GenBank· DDBJ
Genomic DNA
JAALTR010000370
EMBL· GenBank· DDBJ
NGW68747.1
EMBL· GenBank· DDBJ
Genomic DNA
JAANEC010000067
EMBL· GenBank· DDBJ
NUY12192.1
EMBL· GenBank· DDBJ
Genomic DNA
JAANDN010000079
EMBL· GenBank· DDBJ
NUY68722.1
EMBL· GenBank· DDBJ
Genomic DNA
RQSX01000028
EMBL· GenBank· DDBJ
RZH96461.1
EMBL· GenBank· DDBJ
Genomic DNA
QNXM01000003
EMBL· GenBank· DDBJ
TXL50110.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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