W8U685 · W8U685_STAAU
- ProteinProtein/nucleic acid deglycase HchA
- GenehchA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids292 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity
- H2O + N2-(1-hydroxy-2-oxoethyl)-GDP = GDP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GMP = glycolate + GMP + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-GTP = glycolate + GTP + H+
- H2O + N2-(1-hydroxy-2-oxoethyl)-dGTP = dGTP + glycolate + H+
- H2O + N2-(1-hydroxy-2-oxopropyl)-GDP = GDP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GMP = GMP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-GTP = GTP + H+ + lactate
- H2O + N2-(1-hydroxy-2-oxopropyl)-dGTP = dGTP + H+ + lactate
- H2O + N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] = glycolate + H+ + L-lysyl-[protein]
- H2O + N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]
- H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]
- an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
- an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
- an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + H+ + lactate
- an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + H+ + lactate
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 190 | Nucleophile | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | lyase activity | |
Molecular Function | protein deglycase activity | |
Molecular Function | thiolester hydrolase activity | |
Biological Process | DNA repair | |
Biological Process | protein repair |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein/nucleic acid deglycase HchA
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionW8U685
- Secondary accessions
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-23 | Disordered | ||||
Sequence: MSQDVNELSKQPTPDKAEDNAFF | ||||||
Domain | 79-204 | DJ-1/PfpI | ||||
Sequence: PVEMLLPLHHLMEAGFDVDVATLSGYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGHAAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNRE |
Sequence similarities
Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length292
- Mass (Da)32,177
- Last updated2014-05-14 v1
- Checksum7ADA2635287373CD
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP012119 EMBL· GenBank· DDBJ | AKR51333.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP022290 EMBL· GenBank· DDBJ | ATW76519.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACTPC010000001 EMBL· GenBank· DDBJ | CAA4077511.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACTQT010000013 EMBL· GenBank· DDBJ | CAA4389431.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACTSG010000014 EMBL· GenBank· DDBJ | CAA4449620.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACTWD010000014 EMBL· GenBank· DDBJ | CAA4697153.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACTWS010000012 EMBL· GenBank· DDBJ | CAA4776311.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACUKU010000012 EMBL· GenBank· DDBJ | CAA5874430.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACUNS010000014 EMBL· GenBank· DDBJ | CAA6110482.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACUOU010000016 EMBL· GenBank· DDBJ | CAA6139993.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CACURZ010000014 EMBL· GenBank· DDBJ | CAA6376152.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAIGSM010000001 EMBL· GenBank· DDBJ | CAC5478115.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAIGXB010000001 EMBL· GenBank· DDBJ | CAC5778289.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAIHOM010000001 EMBL· GenBank· DDBJ | CAC6998376.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAIIGD010000002 EMBL· GenBank· DDBJ | CAC8204304.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CAIIGN010000009 EMBL· GenBank· DDBJ | CAC8249767.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UAUZ02000002 EMBL· GenBank· DDBJ | CAD7353547.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CSGC01000010 EMBL· GenBank· DDBJ | CPC09472.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CSIU01000013 EMBL· GenBank· DDBJ | CPE21495.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
FJMW01000001 EMBL· GenBank· DDBJ | CZQ26515.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BDVT01000010 EMBL· GenBank· DDBJ | GBV21010.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LALJ01000012 EMBL· GenBank· DDBJ | KMR36711.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LALQ01000066 EMBL· GenBank· DDBJ | KMR56028.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JABXAU010000001 EMBL· GenBank· DDBJ | MBB2557044.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PKSS02000011 EMBL· GenBank· DDBJ | MBW7464080.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WPTS01000002 EMBL· GenBank· DDBJ | MVK33584.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WPXC01000032 EMBL· GenBank· DDBJ | MVM11526.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAAJIY010000015 EMBL· GenBank· DDBJ | NGK21137.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAALWO010000012 EMBL· GenBank· DDBJ | NGV46844.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAALTR010000370 EMBL· GenBank· DDBJ | NGW68747.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAANEC010000067 EMBL· GenBank· DDBJ | NUY12192.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAANDN010000079 EMBL· GenBank· DDBJ | NUY68722.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RQSX01000028 EMBL· GenBank· DDBJ | RZH96461.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QNXM01000003 EMBL· GenBank· DDBJ | TXL50110.1 EMBL· GenBank· DDBJ | Genomic DNA |