W8RNX8 · W8RNX8_9RHOB
- ProteinFlavin-dependent thymidylate synthase
- GenethyX
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids301 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.
Catalytic activity
- dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADPH + H+ = dTMP + (6S)-5,6,7,8-tetrahydrofolate + NADP+
Cofactor
Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.
Pathway
Pyrimidine metabolism; dTTP biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 89 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: T | ||||||
Binding site | 109-112 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: QWIR | ||||||
Binding site | 112-114 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: RHR | ||||||
Binding site | 120 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: E | ||||||
Binding site | 120-124 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: EYSAR | ||||||
Binding site | 196 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain | ||||
Sequence: R | ||||||
Binding site | 212-214 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: DLH | ||||||
Binding site | 218 | FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits | ||||
Sequence: H | ||||||
Active site | 223 | Involved in ionization of N3 of dUMP, leading to its activation | ||||
Sequence: R | ||||||
Binding site | 223 | dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | NADPH binding | |
Molecular Function | thymidylate synthase (FAD) activity | |
Molecular Function | thymidylate synthase activity | |
Biological Process | dTMP biosynthetic process | |
Biological Process | dTTP biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavin-dependent thymidylate synthase
- EC number
- Short namesFDTS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Roseobacteraceae > Roseibacterium
Accessions
- Primary accessionW8RNX8
Proteomes
Interaction
Structure
Sequence
- Sequence statusComplete
- Length301
- Mass (Da)34,194
- Last updated2014-05-14 v1
- Checksum0F6C24D4C25CC6CA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP004372 EMBL· GenBank· DDBJ | AHM02713.1 EMBL· GenBank· DDBJ | Genomic DNA |