W8RNX8 · W8RNX8_9RHOB

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site89FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site109-112dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site112-114FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site120FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site120-124dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site196dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site212-214FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site218FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Active site223Involved in ionization of N3 of dUMP, leading to its activation
Binding site223dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionNADPH binding
Molecular Functionthymidylate synthase (FAD) activity
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-dependent thymidylate synthase
  • EC number
  • Short names
    FDTS
  • Alternative names
    • FAD-dependent thymidylate synthase
    • Thymidylate synthase ThyX
      (TS
      ; TSase
      )

Gene names

    • Name
      thyX
    • ORF names
      roselon_00258

Organism names

Accessions

  • Primary accession
    W8RNX8

Proteomes

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    301
  • Mass (Da)
    34,194
  • Last updated
    2014-05-14 v1
  • Checksum
    0F6C24D4C25CC6CA
MPLTDDQLAEIEAARATPQPTLRAVAPGMEQHLYKAVPVLDHGFVRVIDYMGDDAAIVQAARVSYGAGTKKTRDDSGLIRYLMRHWHSTPFEMCEIKLHVKLPVFVARQWIRHRTANVNEYSARYSILDREFYIPEPDALAAQSTVNNQGRGEVLQGEEAARVLEILKSDAGRAYNNYEAMLSQDGQQGLARELARMNLPANIYTQWYWKVDLHNLFHFLRLRADEHAQYEIRVYAQAIADMVKDWVPAAYGAFEDYRMGGVALSAKGVEVLKRRLAGEDVTQENSGMSKGEWREFVEVFG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP004372
EMBL· GenBank· DDBJ
AHM02713.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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