W8NRU5 · W8NRU5_9EURY
- ProteinN-glycosylase/DNA lyase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids262 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA repair enzyme that is part of the base excision repair (BER) pathway; protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine (GO), from single- and double-stranded DNA substrates.
Catalytic activity
- 2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 43 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 71 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 82 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Active site | 163 | Schiff-base intermediate with DNA | ||||
Sequence: K | ||||||
Binding site | 167 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 193 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Active site | 195 | |||||
Sequence: D | ||||||
Binding site | 229 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 233 | 8-oxoguanine (UniProtKB | ChEBI) | ||||
Sequence: W |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | class I DNA-(apurinic or apyrimidinic site) endonuclease activity | |
Molecular Function | oxidized base lesion DNA N-glycosylase activity | |
Biological Process | base-excision repair |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameN-glycosylase/DNA lyase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Thermococcus
Accessions
- Primary accessionW8NRU5
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Domain
Contains two alpha-helical subdomains, with the 8-oxoguanine binding site located in a cleft at their interface. Contains a helix-hairpin-helix (HhH) structural motif and a Gly/Pro-rich sequence followed by a conserved Asp (HhH-GPD motif).
Sequence similarities
Belongs to the archaeal N-glycosylase/DNA lyase (AGOG) family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length262
- Mass (Da)30,575
- Last updated2014-05-14 v1
- Checksum429DC1B0EC244D48
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP007264 EMBL· GenBank· DDBJ | AHL21948.1 EMBL· GenBank· DDBJ | Genomic DNA |