W7N2B2 · FUB2_GIBM7

Function

function

Part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties (PubMed:22652150, PubMed:25372119).
L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism (By similarity).
The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by the hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by NAD(P)-dependent dehydrogenase FUB6 (By similarity).
FUB1, FUB4, and the non-canonical NRPS Fub8 may form an enzyme complex (By similarity).
Further processing of the NRPS-bound intermediate might be carried out by FUB6 and the sulfhydrylase FUB7, enabling a spontaneous electrocyclization to close the carbon backbone of fusaric acid (By similarity).
Dihydrofusaric acid is likely to be released via reduction by the thioester reductase (TR) domain of FUB8 whereupon the final oxidation to fusaric acid may (also) be performed by the FMN-dependent dehydrogenase FUB9 (By similarity).

Biotechnology

Fusaric acid is phytotoxic to plants such as cotton and banana (PubMed:20955724, PubMed:23922960).
It has been shown to induce programmed cell death in plants (PubMed:16868776, PubMed:23838885).
In addition to a mild toxicity to animals, fusaric acid exhibits acanthamoebicidal, antioomycete, and antimycobacterial activities (PubMed:17927749, PubMed:21811925, PubMed:22864988).

Pathway

Mycotoxin biosynthesis.

Names & Taxonomy

Protein names

  • Recommended name
    Fusaric acid biosynthesis protein 2

Gene names

    • Name
      FUB2
    • ORF names
      FVEG_12522

Organism names

Accessions

  • Primary accession
    W7N2B2

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Does not alter fusaric acid production (PubMed:25372119).

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004373111-104Fusaric acid biosynthesis protein 2

Expression

Induction

Expression is positively regulated by the secondary metabolism regulator LAE1 (PubMed:22713715).

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the YciI family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    104
  • Mass (Da)
    11,683
  • Last updated
    2014-04-16 v1
  • Checksum
    966E2BB50439D7DD
MASELKEYLVIIPDLPDVLAKRQVLLKPHNQDAAPLVKAGRVPFFGSTLAHHSAEGQQVAENGTVMIIKAESEEEIKEIIRKDIFTIEGVWDFGRLSIWPFKSK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000580
EMBL· GenBank· DDBJ
EWG54264.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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