W7MLD8 · FDB95_GIBM7

Function

function

Amidase; part of the Fusarium detoxification of benzoxazolinone cluster 1 (FDB1) involved in the degradation of benzoxazolinones produced by the host plant (PubMed:19302487, PubMed:26808652).
Maize, wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their inherent instability once released, spontaneously degrade to the more stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively (PubMed:11876429).
The first step in the detoxification of benzoxazolinones involves the hydrolysis of the cyclic ester bond of benzoxazolinones by the FDB1 cluster gamma-lactamase MBL1 to aminophenols (PubMed:12788712, PubMed:26808652).
MBL1 is able to convert BOA into 2-aminophenol (2-AP), as well as MBOA into 5-methoxy-2-aminophenol (2-AMP) (PubMed:12788712, PubMed:26808652).
The FDB2 cluster N-malonyltransferase FDB2/NAT1 then metabolizes aminophenols via N-malonylation to non-toxic malonamic acids (PubMed:12788712, PubMed:19302487).
FDB2/NAT1 converts 2-AP into N-(2-hydroxyphenyl) malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl) malonamic acid (HMPMA) (PubMed:12788712, PubMed:19302487).
The duplicated dienlactone hydrolases DLH1 and DLH2 may provide redundant function for hydrolyzing the lactone moiety in the BOA molecule (Probable). The roles of the amidases an other enzymes encoded by the 2 FDB clusters have not been identified so far (Probable)

Miscellaneous

Fusarium verticillioides possesses 2 unlinked loci, FDB1 and FDB2, necessary for detoxification of antimicrobial compounds produced by maize, including 2-benzoxazolinone (BOA) (Probable). The FDB2 cluster arose as a duplication of the FDB1 cluster with rearrangement and expansion by incorporating additional genes (Probable).

Catalytic activity

Pathway

Xenobiotic degradation.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site138Charge relay system
Active site214Charge relay system
Binding site214substrate
Binding site235-238substrate
Active site238Acyl-ester intermediate

GO annotations

AspectTerm
Molecular Functionamidase activity
Molecular Functionindoleacetamide hydrolase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Amidase FVEG_08295
  • EC number
  • Alternative names
    • Fusarium detoxification of benzoxazolinone cluster 1 protein FVEG_08295
      (FDB1 cluster protein FVEG_08295
      )

Gene names

    • ORF names
      FVEG_08295

Organism names

Accessions

  • Primary accession
    W7MLD8

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Does not affect BOA degradation.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004545991-530Amidase FVEG_08295

Expression

Induction

Expression is induced in response to 2-benzoxasolinone (BOA) exposure.

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the amidase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    530
  • Mass (Da)
    58,078
  • Last updated
    2014-04-16 v1
  • Checksum
    720FAAAE761CF41C
MAEVNWQQLINQKRATREALIPEQWLLPATITSKVTPQSTASAFELLSEAGLLTEREIDITEKYTAVSLTAKIATGELSSYDVATAFCKRAAIVHQLTNSLTEIFFDKALERARWLDEYLSKEGKTVGPLHGLPITLKDMIHVKGQYSTMGFVGHLRHPPADENAVITDMLEAAGAVFYCKTNVPQTLFVCESFNNVFGRTLNPYKLCLSPGGSSSGEAAQLGLCGSIMGVGSDIAGSVRVPAIFTGVYGFRPTVNRLPWSKQAELALKGWQGVQPTLGPMARTAQDLTLFMKTIIQAEPWRYDSTALAIPWHDAPRKDKLTIGVWSQDPQFPVFPPIARTMASAVGRLRAAGHTIKIIEAPPTMKAMKIAMRWFALDQVNLPLKFVQDGAESPIADLDAMDPGKFLDPGYVADLSENISISADIHDYREEWAKIWREAGIDVLLCPASRGSAVPHGEFGPLMYTIPWNLLDFPSSVVPFGKADKTLDSKDGYDSTVVDGAPTGFQLVGWRFQDEQTLMATEVIADTLKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM000587
EMBL· GenBank· DDBJ
EWG48584.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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