W7MLD8 · FDB95_GIBM7
- ProteinAmidase FVEG_08295
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids530 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
Amidase; part of the Fusarium detoxification of benzoxazolinone cluster 1 (FDB1) involved in the degradation of benzoxazolinones produced by the host plant (PubMed:19302487, PubMed:26808652).
Maize, wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their inherent instability once released, spontaneously degrade to the more stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively (PubMed:11876429).
The first step in the detoxification of benzoxazolinones involves the hydrolysis of the cyclic ester bond of benzoxazolinones by the FDB1 cluster gamma-lactamase MBL1 to aminophenols (PubMed:12788712, PubMed:26808652).
MBL1 is able to convert BOA into 2-aminophenol (2-AP), as well as MBOA into 5-methoxy-2-aminophenol (2-AMP) (PubMed:12788712, PubMed:26808652).
The FDB2 cluster N-malonyltransferase FDB2/NAT1 then metabolizes aminophenols via N-malonylation to non-toxic malonamic acids (PubMed:12788712, PubMed:19302487).
FDB2/NAT1 converts 2-AP into N-(2-hydroxyphenyl) malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl) malonamic acid (HMPMA) (PubMed:12788712, PubMed:19302487).
The duplicated dienlactone hydrolases DLH1 and DLH2 may provide redundant function for hydrolyzing the lactone moiety in the BOA molecule (Probable). The roles of the amidases an other enzymes encoded by the 2 FDB clusters have not been identified so far (Probable)
Maize, wheat, and rye produce the 2 benzoxazinone phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their inherent instability once released, spontaneously degrade to the more stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), respectively (PubMed:11876429).
The first step in the detoxification of benzoxazolinones involves the hydrolysis of the cyclic ester bond of benzoxazolinones by the FDB1 cluster gamma-lactamase MBL1 to aminophenols (PubMed:12788712, PubMed:26808652).
MBL1 is able to convert BOA into 2-aminophenol (2-AP), as well as MBOA into 5-methoxy-2-aminophenol (2-AMP) (PubMed:12788712, PubMed:26808652).
The FDB2 cluster N-malonyltransferase FDB2/NAT1 then metabolizes aminophenols via N-malonylation to non-toxic malonamic acids (PubMed:12788712, PubMed:19302487).
FDB2/NAT1 converts 2-AP into N-(2-hydroxyphenyl) malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl) malonamic acid (HMPMA) (PubMed:12788712, PubMed:19302487).
The duplicated dienlactone hydrolases DLH1 and DLH2 may provide redundant function for hydrolyzing the lactone moiety in the BOA molecule (Probable). The roles of the amidases an other enzymes encoded by the 2 FDB clusters have not been identified so far (Probable)
Miscellaneous
Fusarium verticillioides possesses 2 unlinked loci, FDB1 and FDB2, necessary for detoxification of antimicrobial compounds produced by maize, including 2-benzoxazolinone (BOA) (Probable). The FDB2 cluster arose as a duplication of the FDB1 cluster with rearrangement and expansion by incorporating additional genes (Probable).
Catalytic activity
- a monocarboxylic acid amide + H2O = a monocarboxylate + NH4+
Pathway
Xenobiotic degradation.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 138 | Charge relay system | ||||
Sequence: K | ||||||
Active site | 214 | Charge relay system | ||||
Sequence: S | ||||||
Binding site | 214 | substrate | ||||
Sequence: S | ||||||
Binding site | 235-238 | substrate | ||||
Sequence: IAGS | ||||||
Active site | 238 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | amidase activity | |
Molecular Function | indoleacetamide hydrolase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAmidase FVEG_08295
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium > Fusarium fujikuroi species complex
Accessions
- Primary accessionW7MLD8
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Does not affect BOA degradation.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454599 | 1-530 | Amidase FVEG_08295 | |||
Sequence: MAEVNWQQLINQKRATREALIPEQWLLPATITSKVTPQSTASAFELLSEAGLLTEREIDITEKYTAVSLTAKIATGELSSYDVATAFCKRAAIVHQLTNSLTEIFFDKALERARWLDEYLSKEGKTVGPLHGLPITLKDMIHVKGQYSTMGFVGHLRHPPADENAVITDMLEAAGAVFYCKTNVPQTLFVCESFNNVFGRTLNPYKLCLSPGGSSSGEAAQLGLCGSIMGVGSDIAGSVRVPAIFTGVYGFRPTVNRLPWSKQAELALKGWQGVQPTLGPMARTAQDLTLFMKTIIQAEPWRYDSTALAIPWHDAPRKDKLTIGVWSQDPQFPVFPPIARTMASAVGRLRAAGHTIKIIEAPPTMKAMKIAMRWFALDQVNLPLKFVQDGAESPIADLDAMDPGKFLDPGYVADLSENISISADIHDYREEWAKIWREAGIDVLLCPASRGSAVPHGEFGPLMYTIPWNLLDFPSSVVPFGKADKTLDSKDGYDSTVVDGAPTGFQLVGWRFQDEQTLMATEVIADTLKA |
Expression
Induction
Expression is induced in response to 2-benzoxasolinone (BOA) exposure.
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length530
- Mass (Da)58,078
- Last updated2014-04-16 v1
- Checksum720FAAAE761CF41C
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000587 EMBL· GenBank· DDBJ | EWG48584.1 EMBL· GenBank· DDBJ | Genomic DNA |