W7DXN2 · W7DXN2_9LIST

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site47Proton acceptor
Binding site67[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site71[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site74[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site119-120S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site151S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site174-176S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site252S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site296S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Probable dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • ORF names
      MCOL2_10655

Organism names

Accessions

  • Primary accession
    W7DXN2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain53-291Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    322
  • Mass (Da)
    36,959
  • Last updated
    2014-04-16 v1
  • Checksum
    703990878B676D7C
MTNVPKESIEILKENFVLNNLEEQIVQKSSDGTIKYLFKLEDGNLIETVLMSHEYGLSVCVTTQVGCNIGCTFCASGLLKKQRDLDAGEIVSQIMNVQRYLDERDNDARVSHIVVMGIGEPFDNYDNVMSFLRVVNHDKGLAIGARHITVSTSGLAPRIKDFANEDFQVNLAVSLHAPNNDIRTKIMRINRTFSIETLMEAIEYYIEKTNRRVTYEYIMLRDVNDSRQEAQELANLLKNHKKLAYVNLIPYNPVDEHNQYERSRKEDVLAFYDVLKKNGVNCVIRREHGTDIDAACGQLRSKQIKRVGIRERMREKKALENQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AODM01000038
EMBL· GenBank· DDBJ
EUJ53459.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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