W6Q0S4 · IFGH_PENRF

Function

function

Fumigaclavine B O-acetyltransferase; part of the gene cluster that mediates the biosynthesis of isofumigaclavines, fungal ergot alkaloids (PubMed:28620689).
The tryptophan dimethylallyltransferase ifgA catalyzes the first step of ergot alkaloid biosynthesis by condensing dimethylallyl diphosphate (DMAP) and tryptophan to form 4-dimethylallyl-L-tryptophan (PubMed:28620689).
The second step is catalyzed by the methyltransferase ifgB that methylates 4-dimethylallyl-L-tryptophan in the presence of S-adenosyl-L-methionine, resulting in the formation of N-methyl-dimethylallyl-L-tryptophan (PubMed:28620689).
The catalase ifgD and the FAD-dependent oxidoreductase ifgC then transform N-methyl-dimethylallyl-L-tryptophan to chanoclavine-I which is further oxidized by ifgE in the presence of NAD+, resulting in the formation of chanoclavine-I aldehyde (PubMed:28902217).
The chanoclavine-I aldehyde reductases ifgG and/or fgaOx3 reduce chanoclavine-I aldehyde to dihydrochanoclavine-I aldehyde that spontaneously dehydrates to form 6,8-dimethyl-6,7-didehydroergoline (PubMed:28620689, PubMed:28902217).
The festuclavine dehydrogenases ifgF1 and/or ifgF2 then catalyze the reduction of 6,8-dimethyl-6,7-didehydroergoline to form festuclavine (PubMed:28620689).
Hydrolysis of festuclavine by a yet undetermined cytochrome P450 monooxygenase (called ifgH) then leads to the formation of isofumigaclavine B which is in turn acetylated by ifgI to isofumigaclavine A (PubMed:28620689).
Penicillium roqueforti has interestingly at least two sets of genes for the consumption of chanoclavine-I aldehyde on three different loci, the OYEs ifgG/fgaOx3 and the festuclavine synthase homologs ifgF1/ifgF2 (PubMed:28620689, PubMed:28902217).
The reason for the duplication of these genes is unclear, probably to ensure the conversion of chanoclavine-I aldehyde by differential gene expression under various environmental conditions (PubMed:28902217).

Catalytic activity

Pathway

Alkaloid biosynthesis; ergot alkaloid biosynthesis.

GO annotations

AspectTerm
Molecular Functionacyltransferase activity
Biological Processindole alkaloid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Fumigaclavine B O-acetyltransferase ifgI
  • EC number
  • Alternative names
    • Isofumigaclavine biosynthesis cluster B protein I

Gene names

    • Name
      ifgI
    • ORF names
      PROQFM164_S02g000302

Organism names

Accessions

  • Primary accession
    W6Q0S4

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004445471-484Fumigaclavine B O-acetyltransferase ifgI

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    484
  • Mass (Da)
    53,739
  • Last updated
    2014-04-16 v1
  • Checksum
    0AFC7D093301C8B3
MSTSFDHSVILSPLDHIAPQAYVSYLLSFQTANSTHCLSLLEAGITRLTKVLPLLLGHIVVNPELDGKYNIQSVQIPCTKEDRTILVHKHHPFPMESALGGVQSGMSMLETSDSKQHLCPLPPLIPSTERQPVIRFQANIFTDAIVLAMTFSHIVFDGTGAAKILALLGRCCRDPSVTPLPLIIDEQDRAQSAIFAGLADTSPAQDHTAELGPAPAIHPVPLDAASLRTCRFEFNSERILQLKYQCSQVLKNACMFQPNSASIPVADLPPFLSSNDVLTSALADAIQRVKSQSKTYDCLDLCMAVNMRGRIELSAAREFLGNMACNLRLKTPGPEYTGPEQCLSCRKTHDCPIQTDQLRFLTDLACKVRNKVRNMDRKYFQSCMTYIANQKDWSQTGMIFTDLAFSSWRHLDIYGLDFGDSFGIVHNFDLSFGLIEGDVIFLPKRLTCDQKEAGWDVHITLPAKDLEALVKDDLIRWLMGRDGE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HG792016
EMBL· GenBank· DDBJ
CDM30153.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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