W5EP13 · CA1P_WHEAT
- Protein2-carboxy-D-arabinitol-1-phosphatase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids495 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phosphoglycerate mutase-like protein lacking PGM activity, but having 2-carboxy-D-arabinitol 1-phosphate (CA1P) phosphatase activity. Can dephosphorylate the closely related compounds 2-carboxy-D-arabinitol 1,5-bisphosphate (CABP) and 2-carboxy-D-ribitol-1,5-bisphosphate(CRBP), and 2,3-diphosphoglycerate. Prevents the accumulation of D-glycero-2,3-pentodiulose-1,5-bisphosphate (PDBP) a potent inhibitor of ribulose-1,5-bisphosphate carboxylase (RuBisCO). PDBP is produced during the oxidation of ribulose-1,5-bisphosphate, the substrate of RuBisCO.
Catalytic activity
- 2-carboxy-D-arabinitol 1-phosphate + H2O = 2-carboxy-D-arabinitol + phosphate
Activity regulation
Inactivated by oxidized glutathione (GSSG) at pH 8.0.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
10 μM | 2-carboxy-D-arabinitol 1-phosphate | |||||
2.9 μM | 2-carboxy-D-arabinitol 1,5-bisphosphate | |||||
4.3 μM | 2-carboxy-D-ribitol-1,5-bisphosphate | |||||
184 μM | 2,3-diphosphoglycerate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
4.2 μmol/min/mg | toward 2-carboxy-D-arabinitol 1-phosphate | ||||
4.3 μmol/min/mg | toward 2-carboxy-D-arabinitol 1,5-bisphosphate | ||||
3.9 μmol/min/mg | toward 2-carboxy-D-ribitol 1,5-bisphosphate | ||||
6.1 μmol/min/mg | toward 2,3-diphosphoglycerate |
pH Dependence
Optimum pH is 7.0.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 58 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Active site | 132 | Proton donor/acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Molecular Function | 2-carboxy-D-arabinitol-1-phosphatase activity | |
Molecular Function | phosphatase activity | |
Biological Process | dephosphorylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-carboxy-D-arabinitol-1-phosphatase
- EC number
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Triticodae > Triticeae > Triticinae > Triticum
Accessions
- Primary accessionW5EP13
- Secondary accessions
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-50 | Chloroplast | ||||
Sequence: MLLFAPTPPPSPATAHRRPGGSAASCIRCSSVRELDRSPSRPPLPPLAEA | ||||||
Chain | PRO_0000430638 | 51-495 | 2-carboxy-D-arabinitol-1-phosphatase | |||
Sequence: KRVVLVRHGQSTWNADGRIQGSSDFSVLTPKGESQAETSRLMLLADSFDACFTSPLARSRRTAEIIWDTRDKDLIPDYDLREIDLYSFQGLLKHEGKEKYGALFQQWQKNPSDCSIDGHYPVRELWDRAQGCWERILTHEGKSVLVVAHNAVNQALVATSLGLGTEYFRTLLQSNCGASVLDFTPQPGGRPPSVCLNRLNQTPSSPISAESSAGRKSSKRIILVCQGATQSSSEGSLGGVGYAPLNMLGVIQAQKTAELLLDLKVNSIICSPQVAAVDTATAICEVQEAAGCLGADCVPRYVEMKNLLGLEIDDAFLTKQKSLEQIVQSGWMGGMEHQKLKTLWAQSEDAWQALVNELPEDDGAESDRVVVAIGHPAIHLGLLCRCLNLTMDYMPSFHLDDGSISVIDFPDGPKGGGIVRCTNYTAHLGRWSVPITKSTENNDEF |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Pro residues | ||||
Sequence: MLLFAPTPPPSPATA | ||||||
Region | 1-23 | Disordered | ||||
Sequence: MLLFAPTPPPSPATAHRRPGGSA |
Sequence similarities
Belongs to the phosphoglycerate mutase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length495
- Mass (Da)53,825
- Last updated2014-03-19 v1
- ChecksumEC7D659257B57DDF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A3B6JFJ7 | A0A3B6JFJ7_WHEAT | CFC21_061271 | 348 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Pro residues | ||||
Sequence: MLLFAPTPPPSPATA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HE603918 EMBL· GenBank· DDBJ | CCE25835.1 EMBL· GenBank· DDBJ | mRNA |