V5VCW7 · V5VCW7_ACIBA
- ProteinMethionine aminopeptidase
- Genemap
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids275 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
Catalytic activity
Cofactor
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )
Note: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 92 | substrate | ||||
Sequence: H | ||||||
Binding site | 110 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 121 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 121 | a divalent metal cation 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 185 | a divalent metal cation 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: H | ||||||
Binding site | 192 | substrate | ||||
Sequence: H | ||||||
Binding site | 218 | a divalent metal cation 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 249 | a divalent metal cation 2 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 249 | a divalent metal cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | initiator methionyl aminopeptidase activity | |
Molecular Function | metal ion binding | |
Molecular Function | metalloaminopeptidase activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine aminopeptidase
- EC number
- Short namesMAP ; MetAP
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Acinetobacter > Acinetobacter calcoaceticus/baumannii complex
Accessions
- Primary accessionV5VCW7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 19-256 | Peptidase M24 | ||||
Sequence: EKMRIAGRLAAEVLDMIKPHIKAGVSTLELDTICRNHIENVQHAIPACVGYGGAPGRPAFQHSICTSVNHVVCHGIPSENKILKNGDILNIDVTVIKDGYHGDTNMMYIVGGETSILANRLCKVAQEAMYRGMATVRDGSYLGDIGHAIQKYVESERFSVVREYCGHGIGTVFHDEPQVLHYGQAGTGMRLEAGMTFTIEPMVNAGVWQTKLLGDKWTVVTKDHKLSAQYEHTILVTK |
Sequence similarities
Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length275
- Mass (Da)30,374
- Last updated2014-02-19 v1
- Checksum6D82AB2AE6DC1802
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP008706 EMBL· GenBank· DDBJ | AKA30982.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP018664 EMBL· GenBank· DDBJ | APP29388.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LN997846 EMBL· GenBank· DDBJ | CUW35909.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
ABFEVW020000005 EMBL· GenBank· DDBJ | EKU3567577.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
ABFEVW030000005 EMBL· GenBank· DDBJ | EMN1070674.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VYVC01000012 EMBL· GenBank· DDBJ | KAA9221432.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LLGC01000179 EMBL· GenBank· DDBJ | KQE03489.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LLGC01000126 EMBL· GenBank· DDBJ | KQE07355.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LRDT01000044 EMBL· GenBank· DDBJ | KZA12740.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACSVK010000003 EMBL· GenBank· DDBJ | MBD0218758.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JACZEI010000027 EMBL· GenBank· DDBJ | MBE0330955.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JARTMM010000087 EMBL· GenBank· DDBJ | MDK4883330.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VMAW01000001 EMBL· GenBank· DDBJ | MDT1909774.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WIOC01000002 EMBL· GenBank· DDBJ | MQR48113.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VYTH01000001 EMBL· GenBank· DDBJ | MQZ66891.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WPIP01000105 EMBL· GenBank· DDBJ | MVM92566.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
WWCH01000001 EMBL· GenBank· DDBJ | MYM79056.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JAAGTY010000009 EMBL· GenBank· DDBJ | NDW41392.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
LYKI01000023 EMBL· GenBank· DDBJ | OIG72371.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NGEL01000129 EMBL· GenBank· DDBJ | OTM85450.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NGKM01000001 EMBL· GenBank· DDBJ | OWK68248.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PHJU02000014 EMBL· GenBank· DDBJ | PQL84274.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PHJT03000020 EMBL· GenBank· DDBJ | PQL88375.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
NEPB01000008 EMBL· GenBank· DDBJ | PRN35987.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
QKWF01000036 EMBL· GenBank· DDBJ | PZM18289.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP042556 EMBL· GenBank· DDBJ | QFH46123.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP061521 EMBL· GenBank· DDBJ | QNV30414.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP059300 EMBL· GenBank· DDBJ | QOJ60316.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP062919 EMBL· GenBank· DDBJ | QPF12406.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP072270 EMBL· GenBank· DDBJ | QTK44638.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RFBY01000023 EMBL· GenBank· DDBJ | RSP76616.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RFCO01000002 EMBL· GenBank· DDBJ | RSQ49839.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
RXYO01000031 EMBL· GenBank· DDBJ | RTY10994.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
SEUG01000001 EMBL· GenBank· DDBJ | RYL46242.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFDJ01000008 EMBL· GenBank· DDBJ | SSI43005.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFMQ01000008 EMBL· GenBank· DDBJ | SST23566.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFNM01000007 EMBL· GenBank· DDBJ | SSU08101.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UFSC01000003 EMBL· GenBank· DDBJ | SUU32078.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VHGY01000042 EMBL· GenBank· DDBJ | TPU61657.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
VHGX01000005 EMBL· GenBank· DDBJ | TPU81270.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP110462 EMBL· GenBank· DDBJ | UZG61185.1 EMBL· GenBank· DDBJ | Genomic DNA |