V4XTM9 · V4XTM9_9ARCH
- ProteinThiamine-monophosphate kinase
- GenethiL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids291 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.
Miscellaneous
Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.
Catalytic activity
- thiamine phosphate + ATP = thiamine diphosphate + ADP
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 20 | Mg2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 20 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 30 | Mg2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 31 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 32 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 32 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 39 | substrate | |||
Binding site | 60 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 60 | Mg2+ 4 (UniProtKB | ChEBI) | |||
Binding site | 60 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 106-107 | ATP (UniProtKB | ChEBI) | |||
Binding site | 107 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 130 | ATP (UniProtKB | ChEBI) | |||
Binding site | 188 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 190 | ATP (UniProtKB | ChEBI) | |||
Binding site | 191 | Mg2+ 5 (UniProtKB | ChEBI) | |||
Binding site | 240 | substrate | |||
Binding site | 287 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | thiamine-phosphate kinase activity | |
Biological Process | phosphorylation | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiamine diphosphate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameThiamine-monophosphate kinase
- EC number
- Short namesTMP kinase ; Thiamine-phosphate kinase
Gene names
Organism names
- Organism
- Taxonomic lineageArchaea > environmental samples
Accessions
- Primary accessionV4XTM9
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length291
- Mass (Da)30,670
- Last updated2014-01-22 v1
- MD5 ChecksumDAB65853C69EFE5C831E1758AD93E337
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KI543230 EMBL· GenBank· DDBJ | ESS11934.1 EMBL· GenBank· DDBJ | Genomic DNA |