V4NRD1 · V4NRD1_9CAUL

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site362Mg2+ (UniProtKB | ChEBI); catalytic
Binding site405Mg2+ (UniProtKB | ChEBI); catalytic
Binding site463Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site466Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      AEAC466_08360

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • AC466
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Caulobacterales > Caulobacteraceae > Asticcacaulis

Accessions

  • Primary accession
    V4NRD1

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, coiled coil, compositional bias.

Type
IDPosition(s)Description
Domain43-178S1 motif
Region105-133Disordered
Coiled coil376-429
Region463-466Required for zinc-mediated homotetramerization and catalytic activity
Region558-679Disordered
Compositional bias568-595Acidic residues
Compositional bias596-610Basic and acidic residues
Compositional bias611-620Basic residues
Compositional bias621-630Basic and acidic residues
Compositional bias636-650Acidic residues
Compositional bias655-666Basic residues
Region733-803Disordered
Compositional bias756-766Basic residues
Region892-922Disordered

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    922
  • Mass (Da)
    102,533
  • Last updated
    2014-01-22 v1
  • MD5 Checksum
    C433F62635B911528D7E51D50212053C
MSKTMLIDSTHTEETRVVVLNGQENGKARVEEFDFESHARKQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQIPVADREKLMAELAAQAQANTNGNGNGHADEDGEDADDDDHGHDDDLPDEERRLRQHLIRRYKIQEVIKRRQILLVQVVKEERGNKGAALTTYMSLAGRYCVLMPNTARGGGISRKITNAADRKRLKTLVTTLDVPHGMGLIVRTAGAKRTKLEIKRDYDYLLRVWETIRETTLRSNAPALIYEEEDLVKRAIRDLFDKDFAGVLVEGEEGYKSARDFMRMIMPSQAKKIQLYKANIPLFARYGVEELISKIYSPVVPLKSGGYLVINQTEALVAIDVNSGKSTKERNIEATALKTNMEAAEESARQMRLRDLAGLVVIDFIDMDEAKNNRSVEKKLKDALSEDRARIQMGKISSFGLMEISRQRRRTGFLEGTTVVCPHCEGQGRIRSVDSAALSAMRVIDMEAMQNGAGAITVKVPMDIALYILNEKQEHLAKLRGEWGLKVNIEIDTDLGHTEVQIVRTVHADEVDFVPPVRPEPIAFDMSDEVVEEEEDDDDEVAIEREDTDDDEASERLETRVEGRDDRRGKRRRRRRGGRERPEAEDRAAAPAGEGDEDGEEEMDGEVGEDGRRGRRRRGRRGGRRAGFDSRPRETYSWVRARTPSLEAPYVWIDPFEEQQRREHPRSEMAHQPLIAAEASDVSVAQAVVSALGAESTSPSSIVPEALRGQPAPKRQRPPRRRGKGGVDAADPVAVNASEVVITAPMPTSTDVDTGVAPELTPQSDPAIAVLAPVDVTAEVAEMAKPVRKTRSRKKVVDETAETKSADIAPEAIASVPAPVAPKPKAARGRKAAAPVAVEALVEDVITVSLPEPEPQPVVAPDPNEISAPPEKPKRGWWRR

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias568-595Acidic residues
Compositional bias596-610Basic and acidic residues
Compositional bias611-620Basic residues
Compositional bias621-630Basic and acidic residues
Compositional bias636-650Acidic residues
Compositional bias655-666Basic residues
Compositional bias756-766Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AWGE01000007
EMBL· GenBank· DDBJ
ESQ84357.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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