V4MH95 · V4MH95_EUTSA

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by AMP.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site101ATP (UniProtKB | ChEBI)
Binding site164-165ATP (UniProtKB | ChEBI)
Binding site189-192ATP (UniProtKB | ChEBI)
Binding site190Mg2+ (UniProtKB | ChEBI); catalytic
Site191Important for substrate specificity; cannot use PPi as phosphoryl donor
Binding site218-220substrate
Active site220Proton acceptor
Binding site263-265substrate
Binding site319substrate
Binding site375-378substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processroot epidermal cell differentiation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent 6-phosphofructokinase
  • EC number
  • Short names
    ATP-PFK
    ; Phosphofructokinase
  • Alternative names
    • Phosphohexokinase

Gene names

    • Name
      PFK
    • ORF names
      EUTSA_v10025029mg

Organism names

Accessions

  • Primary accession
    V4MH95

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain95-399Phosphofructokinase
Region451-489Disordered

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    489
  • Mass (Da)
    53,611
  • Last updated
    2014-01-22 v1
  • Checksum
    FE8FF99B3823DAFD
MSTVESSKPKIINGSCGYVLEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDGPRGIHFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAKNTVSLDSKAVNDIHKRGGTILGTSRGGHDTVKIVDSIQDRGINQVYIIGGDGTQRGASVIFEEIRRRGLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESMENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDYFNQKKMVMNLKYIDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYVSGLVNGRQTYIPFYRITEKQNHVVITDRMWARLLSSTNQPSFLSPKDVSDDKEQPMSALLDDGNCNGSVDVPPVTKEITK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KI517384
EMBL· GenBank· DDBJ
ESQ54662.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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