V4LIW1 · V4LIW1_EUTSA
- ProteinNAD(P)H-hydrate epimerase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids533 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.
Catalytic activity
- (6R)-NADHX = (6S)-NADHX
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 potassium ion per subunit.
Pathway
Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.
Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 134-138 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: NNGGD | ||||||
Binding site | 135 | K+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 199 | K+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 203-209 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: GFSFHGA | ||||||
Binding site | 241 | (6S)-NADPHX (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 244 | K+ (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | FMN binding | |
Molecular Function | metal ion binding | |
Molecular Function | NADHX epimerase activity | |
Molecular Function | NADPHX epimerase activity | |
Molecular Function | pyridoxamine phosphate oxidase activity | |
Biological Process | NADH metabolic process | |
Biological Process | NADP metabolic process | |
Biological Process | pyridoxine biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD(P)H-hydrate epimerase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Eutremeae > Eutrema
Accessions
- Primary accessionV4LIW1
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 84-300 | YjeF N-terminal | ||||
Sequence: AAEIDETLMGPLGFTVDQLMELAGLSVATSIAEVYKPTEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPSICYPKRTDKSLYTGLVTQLESLSIPFVSVDDLPEDLSKDFDVIIDAIFGFSFHGAPRPPFDDLIRRLVSLQNSEKTLQKHPVIASVDIPSGWHVEEGDHEGEGIKPDMLVSLTAPKLCAKRFCGPHHFLGGRFVPPQVAEKYKLEL |
Sequence similarities
Belongs to the NnrE/AIBP family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length533
- Mass (Da)59,842
- Last updated2014-01-22 v1
- ChecksumC47750C75D4DBA6B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KI517464 EMBL· GenBank· DDBJ | ESQ43654.1 EMBL· GenBank· DDBJ | Genomic DNA |