U6G9T9 · U6G9T9_EIMAC
- ProteinProbable ATP-dependent 6-phosphofructokinase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1452 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 307 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 371-372 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RG | ||||||
Binding site | 400-403 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GDGS | ||||||
Binding site | 401 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Site | 402 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 429-431 | substrate | ||||
Sequence: TID | ||||||
Active site | 431 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 476-478 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 537 | substrate | ||||
Sequence: E | ||||||
Binding site | 634-637 | substrate | ||||
Sequence: YEGR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process | |
Biological Process | response to glucose |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Apicomplexa > Conoidasida > Coccidia > Eucoccidiorida > Eimeriorina > Eimeriidae > Eimeria
Accessions
- Primary accessionU6G9T9
Proteomes
Organism-specific databases
Subcellular Location
Interaction
Subunit
Tetramer of two alpha (regulatory) and two beta (catalytic) chains.
Structure
Family & Domains
Features
Showing features for region, domain, coiled coil, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-60 | Disordered | ||||
Sequence: MPGERLPLGGQGAHKTTNAPPLPAHRSPSGRPGGGALPTGAGGAAGAAAAAGGVGAGVGL | ||||||
Region | 80-139 | Disordered | ||||
Sequence: KLSAPPADPHGARSPSRDAWRPTITTGPAQSPKTPGERELVAPASGAPQAARQQQGSRPV | ||||||
Domain | 299-552 | Phosphofructokinase | ||||
Sequence: RIGIVLSGGPAPGGHNVIAGLYDFVKQKHPESMLVGFMGGLDGVMNQNYTVVTEELMEDFRNQGGFDMLWSGRGRVGGEEDIQRAREVCESLELHGFVLVGGDGSNSNAALLAESFEKDLPSCCVIGVPKTIDGDLKNALIEASFGFDTAAKTYSELIGNLCIDVNSSQKVYHFVRVMGRSASHLVLECAMQARPNLVFIGEEVERENMSLEHIVQELVDLVVKRAEQGKMYGVILVPEGLIEFIPEMKQLIKE | ||||||
Domain | 866-1049 | Phosphofructokinase | ||||
Sequence: FGLTGLIEKNYIEITDENLELFLNQGGCELTGRSTSHCLGSVENQEAVRQRCEELMLDGLVMPGSAFAMSEAALVAEYFLARQCRTSIIGIPATGSNNLAGEFIETCVGFDSSTKVYAALIGNVLTDAASMPKYWHFIRLMGRQPSFEVLECALQTHPNVVIIAEEYGAADKTLLHVVQDIADV | ||||||
Coiled coil | 1073-1100 | |||||
Sequence: LPNMKILLAELRRIIREAQAKGEMKKAQ | ||||||
Region | 1268-1288 | Disordered | ||||
Sequence: TGTSAADGVMDRTSRTPSVTE | ||||||
Region | 1411-1452 | Disordered | ||||
Sequence: ALGQSDETDFTDVEPAALRKTTWRDQGGQLGRPSSSSKHPGN | ||||||
Compositional bias | 1437-1452 | Polar residues | ||||
Sequence: GGQLGRPSSSSKHPGN |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,452
- Mass (Da)159,809
- Last updated2014-01-22 v1
- Checksum7717A352410DE03D
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1437-1452 | Polar residues | ||||
Sequence: GGQLGRPSSSSKHPGN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
HG670489 EMBL· GenBank· DDBJ | CDI76905.1 EMBL· GenBank· DDBJ | Genomic DNA |