U5TBE3 · U5TBE3_COWPX
- ProteinCPXV197 protein
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids504 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Nuclease that is responsible for viral evasion of host cGAS-STING innate immunity. Cleaves 2',3'-cGAMP which is produced by host cGAS following recognition of cytosolic DNA and blocks the subsequent 2',3'-cGAMP-mediated activation of TMEM173/STING, which normally spreads to adjacent cells and activates the interferon and NF-kappa-B immune responses.
Catalytic activity
- 2',3'-cGAMP + H2O = Gp(2'-5')Ap(3') + H+This reaction proceeds in the forward direction.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 17 | Proton donor | ||||
Sequence: H | ||||||
Site | 60 | Substrate binding | ||||
Sequence: R | ||||||
Site | 105 | Substrate binding | ||||
Sequence: I | ||||||
Active site | 138 | Shared with catalytic histidine of dimeric partner | ||||
Sequence: Y | ||||||
Active site | 142 | Proton acceptor; shared with catalytic histidine of dimeric partner | ||||
Sequence: K | ||||||
Site | 149 | Substrate binding | ||||
Sequence: N | ||||||
Site | 169 | Substrate binding | ||||
Sequence: Q | ||||||
Site | 182 | Substrate binding | ||||
Sequence: R | ||||||
Site | 184 | Substrate binding | ||||
Sequence: R | ||||||
Site | 186 | Substrate binding | ||||
Sequence: K |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | 2',3'-cyclic GMP-AMP binding | |
Molecular Function | nuclease activity | |
Biological Process | symbiont-mediated suppression of host innate immune response |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Nucleocytoviricota > Pokkesviricetes > Chitovirales > Poxviridae > Chordopoxvirinae > Orthopoxvirus
- Virus hosts
Accessions
- Primary accessionU5TBE3
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 244-356 | Poxin-Schlafen/Schlafen-like N-terminal | ||||
Sequence: SIRHLWSVVYDHFDVVNGEERCYVHMHSSNQSPMLSTVKTNLYMKTMGACLQMDSMEALEYLSELKESGGRSPRPELQKFEYPDGVEDAGSIERLAEEFFSRSELQAGESVKF | ||||||
Domain | 374-484 | Schlafen AlbA-2 | ||||
Sequence: TRIRQQLPSILSSFANTDGGYLFIGVDNNTHKVVGFTVGQDYLKLVESEIEKRIRRLHVVHFCEKKEDIKYACRFIKVYKPGEETTSTYVCAIKVERCCCAVFADWPESWY |
Domain
The substrate binding site is formed by the N-terminus of a monomer and the C-terminus of the opposite monomer.
Family and domain databases
Sequence
- Sequence statusComplete
- Length504
- Mass (Da)57,202
- Last updated2014-01-22 v1
- Checksum96570EB5E73536B7
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
KC813496 EMBL· GenBank· DDBJ | AGY98136.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035751 EMBL· GenBank· DDBJ | AZY89890.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035752 EMBL· GenBank· DDBJ | AZY90075.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035754 EMBL· GenBank· DDBJ | AZY90444.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035755 EMBL· GenBank· DDBJ | AZY90629.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035756 EMBL· GenBank· DDBJ | AZY90819.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
MK035758 EMBL· GenBank· DDBJ | AZY91192.1 EMBL· GenBank· DDBJ | Genomic DNA |