U5GXS3 · U5GXS3_USTV1
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1192 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 155 | ATP (UniProtKB | ChEBI) | |||
Binding site | 239 | ATP (UniProtKB | ChEBI) | |||
Binding site | 274 | ATP (UniProtKB | ChEBI) | |||
Active site | 330 | ||||
Binding site | 585 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 657 | substrate | |||
Binding site | 755 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 785 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 787 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 920 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Pucciniomycotina > Microbotryomycetes > Microbotryales > Microbotryaceae > Microbotryum
Accessions
- Primary accessionU5GXS3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 755 | N6-carboxylysine | |||
Modified residue | 1156 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 9-34 | Disordered | |||
Domain | 39-488 | Biotin carboxylation | |||
Domain | 159-355 | ATP-grasp | |||
Domain | 576-846 | Pyruvate carboxyltransferase | |||
Domain | 1112-1190 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,192
- Mass (Da)129,997
- Last updated2013-12-11 v1
- MD5 ChecksumD03E33F356E8CB50AA1ED9DA146DDF45
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
U5GXS4 | U5GXS4_USTV1 | MVLG_00001 | 1129 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AEIJ01000001 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
GL541643 EMBL· GenBank· DDBJ | KDE09592.1 EMBL· GenBank· DDBJ | Genomic DNA |