T0Q2R4 · T0Q2R4_SAPDV
- Protein3'-phosphate/5'-hydroxy nucleic acid ligase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids430 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
Catalytic activity
- a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate
Cofactor
Note: Binds 2 manganese ions per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 72 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 187-191 | GMP (UniProtKB | ChEBI) | |||
Binding site | 188 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 206 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 300 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 300-301 | GMP (UniProtKB | ChEBI) | |||
Active site | 350 | GMP-histidine intermediate | |||
Binding site | 350-353 | GMP (UniProtKB | ChEBI) | |||
Binding site | 428 | GMP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA ligase activity | |
Molecular Function | GTP binding | |
Molecular Function | manganese ion binding | |
Molecular Function | RNA ligase (GTP) activity | |
Biological Process | DNA repair | |
Biological Process | RNA processing | |
Biological Process | RNA repair |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3'-phosphate/5'-hydroxy nucleic acid ligase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Sar > Stramenopiles > Oomycota > Saprolegniales > Saprolegniaceae > Saprolegnia
Accessions
- Primary accessionT0Q2R4
Proteomes
Organism-specific databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length430
- Mass (Da)46,181
- Last updated2013-10-16 v1
- Checksum1F83C5F624352F60
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH767165 EMBL· GenBank· DDBJ | EQC32129.1 EMBL· GenBank· DDBJ | Genomic DNA |