T0KMW4 · T0KMW4_COLGC

Function

function

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Pathway

tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.

Features

Showing features for binding site.

140050100150200250300350400
TypeIDPosition(s)Description
Binding site57-59ATP (UniProtKB | ChEBI)
Binding site63ATP (UniProtKB | ChEBI)
Binding site89ATP (UniProtKB | ChEBI)
Binding site167ATP (UniProtKB | ChEBI)
Binding site172ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionnucleotidyltransferase activity
Molecular FunctiontRNA binding
Biological Processprotein urmylation
Biological ProcesstRNA thio-modification
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytoplasmic tRNA 2-thiolation protein 1
  • EC number
  • Alternative names
    • Cytoplasmic tRNA adenylyltransferase 1

Gene names

    • Name
      NCS6
    • Synonyms
      CTU1
    • ORF names
      CGLO_02893

Organism names

Accessions

  • Primary accession
    T0KMW4

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain53-233tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal
Domain368-398Cytoplasmic tRNA 2-thiolation protein 1 C-terminal

Sequence similarities

Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    400
  • Mass (Da)
    43,420
  • Last updated
    2013-10-16 v1
  • Checksum
    C4CF42C2D9894067
MAPVLCANCKTERALVKRPKNHQKLCKACFISVFEEEVHHTIVSSALFAAGEKVAIGASGGKDSTVLANVLKTLNERYSYGLELVLLSIDEGIKGYRDDSLETVKRNAVQYDMLLTVVGYDQLYGWTMDQVVETIGKKGNCTYCGVFRRQALDRGAKMLGIKHLVTGHNADDVAETILMNLLRGDLPRLGRSTSITTGDDSSDVKRSKPLKYSYEKEIVLYAHHQKLDYFSTECIYSPEAFRGTARTLIKNLERVRPSAILDIVRSGEDMGRLVAGASSNSCACKGDSGRIAEDDYASGCGSQNGNGSKGEIARLDAQLSQTTDDADLVTDYTTAVAGKKEPHADIVVGQPKPSTQQAATNGPLQKLGSCERCGYMSSQAVCQACLLVDGLNKNKAEIRI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AMYD01000595
EMBL· GenBank· DDBJ
EQB57032.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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