T0KMW4 · T0KMW4_COLGC
- ProteinCytoplasmic tRNA 2-thiolation protein 1
- GeneNCS6
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids400 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm5 tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.
Pathway
tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | protein urmylation | |
Biological Process | tRNA thio-modification | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytoplasmic tRNA 2-thiolation protein 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Glomerellales > Glomerellaceae > Colletotrichum > Colletotrichum gloeosporioides species complex
Accessions
- Primary accessionT0KMW4
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 53-233 | tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal | ||||
Sequence: KVAIGASGGKDSTVLANVLKTLNERYSYGLELVLLSIDEGIKGYRDDSLETVKRNAVQYDMLLTVVGYDQLYGWTMDQVVETIGKKGNCTYCGVFRRQALDRGAKMLGIKHLVTGHNADDVAETILMNLLRGDLPRLGRSTSITTGDDSSDVKRSKPLKYSYEKEIVLYAHHQKLDYFSTE | ||||||
Domain | 368-398 | Cytoplasmic tRNA 2-thiolation protein 1 C-terminal | ||||
Sequence: GSCERCGYMSSQAVCQACLLVDGLNKNKAEI |
Sequence similarities
Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length400
- Mass (Da)43,420
- Last updated2013-10-16 v1
- ChecksumC4CF42C2D9894067
Keywords
- Technical term