S9UXD9 · S9UXD9_9TRYP
- ProteinPutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.
Catalytic activity
- cytidine32/guanosine34 in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine32/2'-O-methylguanosine34 in tRNA + 2 H+ + 2 S-adenosyl-L-homocysteine
RHEA-COMP:10246 CHEBI:74269 Position: 34CHEBI:82748 Position: 32+ 2 CHEBI:59789 = RHEA-COMP:10247 CHEBI:74445 Position: 34CHEBI:74495 Position: 32+ 2 CHEBI:15378 + 2 CHEBI:57856
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 56 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 58 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 90 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 106 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 142 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 182 | Proton acceptor | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | tRNA (cytidine(32)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (guanine(34)-2'-O)-methyltransferase activity | |
Biological Process | cytoplasmic translation | |
Biological Process | tRNA nucleoside ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Strigomonadinae > Strigomonas
Accessions
- Primary accessionS9UXD9
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 21-225 | Ribosomal RNA methyltransferase FtsJ | ||||
Sequence: YRARSAYKLLQIHEDYHILDPAQVQYGAVDLCAAPGSWSQVLSSILACPPPGDVPASPAAYVPPPVIAVDLQEMAPIPHVTILQGDITSTTTAQQILDLLAAANRRRGARSADARASIVVCDGAPDVTGLHELDEYLQHQLLLAALLITTCVLRPGGTFVTKMFRGPNTPFLVHKSELFFEEVRVVKPKSSRNASVESFLLCQRF | ||||||
Region | 304-372 | Disordered | ||||
Sequence: DEEHTEGGGGAAPHPAGRRAKGHVLPPVQPPLQAPYLPPPPPAPRTRDETDGSARSTSPKESPTKKTKQ | ||||||
Compositional bias | 331-349 | Pro residues | ||||
Sequence: VQPPLQAPYLPPPPPAPRT | ||||||
Compositional bias | 351-372 | Basic and acidic residues | ||||
Sequence: DETDGSARSTSPKESPTKKTKQ |
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)40,102
- Last updated2013-10-16 v1
- ChecksumADF8B5CC11301C70
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 331-349 | Pro residues | ||||
Sequence: VQPPLQAPYLPPPPPAPRT | ||||||
Compositional bias | 351-372 | Basic and acidic residues | ||||
Sequence: DETDGSARSTSPKESPTKKTKQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ATMH01012431 EMBL· GenBank· DDBJ | EPY15195.1 EMBL· GenBank· DDBJ | Genomic DNA |