S7ZZD2 · S7ZZD2_DACHA
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1195 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 154 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 238 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 273 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 330 | |||||
Sequence: R | ||||||
Binding site | 584 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 656 | substrate | ||||
Sequence: R | ||||||
Binding site | 752 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 782 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 784 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 917 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Orbiliomycetes > Orbiliales > Orbiliaceae > Dactylellina
Accessions
- Primary accessionS7ZZD2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 752 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1153 | N6-biotinyllysine | ||||
Sequence: K |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 36-488 | Biotin carboxylation | ||||
Sequence: QLKKIMVANRGEIPIRIFRTAHELSLQTVAIYSYEDRMGMHRQKADEAYMIGHRGQYTPVGAYLAIDEIIKIALEHNVNMIHPGYGFLSENAEFARKVEAAGIIFVGPTYKVIEALGDKVSARKIALQCNVPCVPGTPGPVESFDDCSTFVKEYGYPIIIKAAFGGGGRGMRVVREGDNLQDAFERATSEAKSAFGNGTVFIERFLDKPKHIEVQLLGDNQGNVVHLYERDCSVQRRHQKVVEIAPAKDLPPETRQAILDDAVRIAKSVNYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGATLAQLGLTQDRISTRGFAIQCRVTTEDPSENFRPDTGKIEVYRSAGGNGVRLDGGNGFAGSIITPHYDSMLVKCTCHGSTYEIARRKVIRALVEFRIRGVKTNIPFLLSLLTHEVFINGTCWTTFIDDTPE | ||||||
Domain | 158-355 | ATP-grasp | ||||
Sequence: RKIALQCNVPCVPGTPGPVESFDDCSTFVKEYGYPIIIKAAFGGGGRGMRVVREGDNLQDAFERATSEAKSAFGNGTVFIERFLDKPKHIEVQLLGDNQGNVVHLYERDCSVQRRHQKVVEIAPAKDLPPETRQAILDDAVRIAKSVNYRNAGTAEFLVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAA | ||||||
Domain | 575-843 | Pyruvate carboxyltransferase | ||||
Sequence: CLLMDTTWRDAHQSLLATRVRTIDICEIAKHTSYAYSNAYSIECWGGATFDVAMRFLYEDPWDRLRKMRRLVPNVPFQMLLRGANGVAYSSLPDNAIEHFCEQARKNGVDIFRVFDALNDVDQLEVGIKAVKKAGGVVEAAICYSGDMLNPTKKYNLEYYLELAEKVVKLGTHILGIKDMAGCLRPRAATLLIGALREKYPDLPIHVHTHDSAGTGVASMAACAAAGADAVDTATDSLSGMTSQPSVGAVLAALEGTDMDPGLDINQVR | ||||||
Domain | 1112-1187 | Lipoyl-binding | ||||
Sequence: KANQADSSQVGAPMSGVVVEVRVKDGSEVKKGDPIAVLSAMKMEMVISAPHSGVIAELQIKANDSVDGQDLICKIV |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,195
- Mass (Da)131,429
- Last updated2013-10-16 v1
- Checksum98A4A964F386F564
Keywords
- Technical term