Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

S7R7E3 · S7R7E3_GLOTA

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Catalytic activity

  • (2E)-decenoyl-[ACP] + NADPH + H+ = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + NADPH + H+ = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + NADPH + H+ = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + NADPH + H+ = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + NADPH + H+ = octadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + NADPH + H+ = octanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + NADPH + H+ = tetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + NADPH + H+ = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • octanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • tetradecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + malonyl-[ACP] + H+ = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.

Pathway

Lipid metabolism.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site946Proton acceptor; for dehydratase activity
Active site1118Proton donor; for dehydratase activity

GO annotations

AspectTerm
Molecular Function(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Function[acyl-carrier-protein] S-malonyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Molecular Functionphosphopantetheine binding
Biological Processfatty acid biosynthetic process
Biological Processphenol-containing compound biosynthetic process
Biological Processpolyketide biosynthetic process
Biological Processtoxin biosynthetic process

Keywords

Names & Taxonomy

Protein names

  • Submitted names
    • Polyketide synthase

Gene names

    • Name
      pks6
    • ORF names
      GLOTRDRAFT_123623

Organism names

Accessions

  • Primary accession
    S7R7E3

Proteomes

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain14-438Ketosynthase family 3 (KS3)
Region906-1041N-terminal hotdog fold
Domain906-1213PKS/mFAS DH
Region1059-1213C-terminal hotdog fold
Domain2267-2345Carrier

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,640
  • Mass (Da)
    286,543
  • Last updated
    2013-10-16 v1
  • MD5 Checksum
    A3DE715EB05560243C0C53E33620C2B8
MVSQVAPSYSNPDVRALAVVGMSICAPGGEESPRGLDVDAFQEFLATRGDPMIVVPKDRWNAEAFHGSQPGKLVTTKGGFIPEISYGDLSEFGVTPAEAAQVGTPQLVLLHQAFNALQRSGVDYRHTSTGVYIGVGGSGVPYEMDITRAGAYYSTGMAMSIAANRINYVFDLMGPSVPVDTACSSSLVAIHLAIQAIRNGECEQAVIAGVNFVSNPLDTVALSQLGLLSEDGHCKSFSDDADGCARADVVNAVVIKRHDLAVRDHDKILASLVGTSLTTCGNIMGSLTTPNPEAHIMAIRAAYDDAGLQPHQVDFLELHGTGTPVGDMLETNAAGATFSEGRNGRELIIGSVKSNVGHTETGSYLTALVKVIMMLQAKQILPNGYFKTPSKKIHFDEYKLRVPTAPEEFKAHDKELGRIVSISNFGYGGAGGHTVIREHEERKPLPDLEALRKGPFLFTMGALSPRALQTLVSTYKEQYQNVDPLALAEHLGKRTRQMPWRTFAVADSLAAASFPEAVMVDKLPSPVVFTFSGQGPQHWNQGRLLFQTYSVFRESILACDDVHQEYTGRSFLEDTGLFINGKSSELEKSLVWPALTISVAITFFQVAMTDLLTSLNILPKAVVGHSIGETAVLYASGAMPKSVVVKVAIARGKALLLVDNIGGAMVAISGTDNNSLQDYVEAVSALAQDQGKDAKHLYLATFNSPTDIGVSGSDALVDMLANYINTWADGATARKLRVSTAVHSPFVDICEKSYRAELKSIFSKHKGPFVPKVPTMSTVTGEFVDGEYTIDYLWKNLRQAVLFSTAIPKLIERFGENTVFLEVSPHPVLSSYIEQMGASSAFACSIRPPSARQIASGAKNTNELQAFLQAVGRLLVSGVNSINFSVLNGCPSETITGPAYPFQWKHTPVAPRLPSYLSCLLPPTRLLNSSRLRVSPKLPEEWMGDHIVDETNLVPASAYIEMALEFDGVTQVWDCRFDAPCILDENASPATLEVAKDGNRFTIKSSSSLESMQGDLEWTRSGPVFDTLHATGKLGYGKPVTGPNAVTKIDVQDVLSRCFARYTKEEIYAEMHDILQFGPEFSRVERAALNEDEVVCWIRGHVEGVNAKDYAFHPAIMDAAFQSGNVFNLLYDKIPTGRDGRVLYLPYTLRRGYRNDGSTGPLVLPAEFKTYTKLAEWTTDHFTLDIYVLGENDEVVYTFEGKFPEFQPTLTLTQHCHAGLQLNFLNKDTVWPTERYMNYWQPTALPPSRFEGVNDTLDTRVDASEAIQLLKMLDYRAMKSTAQTLKDVPGNFSPDAYDRQRYLAWAKIQAGRFPTVPPVGSDEAIEERYADLFELVDRIAPVQKDILRTSTAAVEVMFRDNIMSKIYELPPFVGDVFGEVATKFVELVKNAVAAGKKVVRVLEVGAGTGRLTALLGQALIDARIQDLCYVDYVSTDISISLAQESTVKSPWPTMTPKALDLSVPLAQQGFDANTFDIVTAFDVLHAIPDIGSTLAALKELLVPGGHIAIIELDGRRFAQEAPGTIYMDYVFGSFAEWFGVLQHRENGSHCSLTPEQWKVALTDAGFGDNLFLSSPGSQIIHMAFVSQGPKTVYTGAYVPPTPNAYTLRGYKAGEEIDLVNFVSTLDSQKPHTLWLYADTDNIKLLGIARSIRQEFGLWKIMLVIFDPSWSTEQRERFIQEKLVMLPYLDSEVQVDADGNLKVPRVIKAPSPPQTEPAQSKPLAFDGKTVWRAYPQPVGAHDVEVAVDYVNVRPLFSDVTEFTGVVTAVGSDTSDGALVGKRVVGIAYKPPSTVIVCPRGQVSPVEGEATSSLAVGPRAAFISSIVQPLVSSITAGDSHVLVHVGSGGPAALAVVKYLQSLKAKVFVTASDPSTLVLAGISTPAVRSTEYDVWVTASQAWAPHGIEIAFNFDNDRNIVAETRELLSKTGTLVDATGRFPVGLKNSQRYIAVDSTSVINASRLIPEGLSSLSKEALEALSPKVAVFTLPQLGDATHATAAEDTAVLLDVKSETDSLSVTRAGVLKGTNAFDPRATYIVVGGSLKPGKLVYQKKILQSLRSVPGVTIDIVANDIMDLEQAKALFSGERRVAGVFFLPVVLEDTLFANMTSEKYWKPVTDVKVKGLTNLVQAKNPAELDFLVIASTAAILLGSEGQANYTSANVQMEQLAAKLPNTVTISVPALTDGGVYVNSIAKNQSRNAALDKFRDLGYSTLRVAEHCVDAIWTLNTPAYNPLYIPPLVWRGVMDVVPPDNRPFVRHLLVKESSENRAADGAKESSIRGTCASVLSMEVDELEDIVPLASYGLDSLTSVRLSGLLQQKFGIAVTQMQLLSSHMTVKKLEEMQEEQNAVASPAAAVNGQVNGDGGAALGDDVSQTVVKLNGVADGTPLFVLHGAGGGISVMRKFAQKVKCPVYGVQDTPEAPINGTIYTLAQFYTAKIREKQPHGPYHLAGFSFGTYVVLYVAMELRDAGEVVAPLIMIDSAPTLFIREEFRAWSSKNLLDNKLKEEIMGVVTDMSTSGSLDDTEEVIGQFEEHFKKKERGEAGAKWVARFCRAYAAHFIMSIRASKDLAEEMKKPWPSERTVLIRAQGGVATKPQFKGASRALGLDKWAAHVDVHELPGTHFGILNPDNGIADVLNGVCGV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB469319
EMBL· GenBank· DDBJ
EPQ50300.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help