S7J231 · S7J231_9FIRM
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1144 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 119 | ATP (UniProtKB | ChEBI) | |||
Binding site | 203 | ATP (UniProtKB | ChEBI) | |||
Binding site | 238 | ATP (UniProtKB | ChEBI) | |||
Active site | 295 | ||||
Binding site | 541 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 613 | substrate | |||
Binding site | 710 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 739 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 741 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 874 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Negativicutes > Veillonellales > Veillonellaceae > Megasphaera
Accessions
- Primary accessionS7J231
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 710 | N6-carboxylysine | |||
Modified residue | 1109 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-456 | Biotin carboxylation | |||
Domain | 123-320 | ATP-grasp | |||
Domain | 532-800 | Pyruvate carboxyltransferase | |||
Domain | 1074-1144 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,144
- Mass (Da)127,790
- Last updated2013-10-16 v1
- ChecksumC88FC30FC52EFB8D
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
APHY01000027 EMBL· GenBank· DDBJ | EPP18001.1 EMBL· GenBank· DDBJ | Genomic DNA |