S5UH55 · TRPM5_DANRE

Function

function

Monovalent cation-selective ion channel activated by intracellular Ca2+ in a voltage- and temperature-dependent manner. Mediates the transport of Na+, K+ and Cs+ ions equally well. Activated directly by increase in intracellular Ca2+, but is impermeable to it. The activation mechanism of TRPM5 involves a multistep process. TRPM5 activation involves ligand binding (i.e., tastant molecule, glucose stimulation) to Gq/G-protein coupled receptors (GPCR) and leads to the breakdown of phosphatidylinositol bisphosphate (PIP2) into diacylglycerol (DAG) and inositol trisphosphate (IP3), IP3 binds to its receptors in the endoplasmic reticulum and cause Ca2+ release. Simultaneously with the intracellular Ca2+ release, DAG activates the protein kinase C (PKC), which phosphorylates the TRPM5 channel. This phosphorylation combined with the bound Ca2+, leads to a robust inward current allowing the entry of sodium ions (Na+) into the cell. This ion influx depolarizes the cell membrane, generating action potentials that propagate TRPM5 signals.

Catalytic activity

Activity regulation

Ca2+-activated cation channel (PubMed:34168372).
Displays voltage dependence modulation (PubMed:34168372).
Regulated by PI(4,5)P2 levels. PI(4,5)P 2 reverses the Ca2+ -induced desensitization of channels. Is highly temperature-sensitive (By similarity).

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site212Ca2+ 1 (UniProtKB | ChEBI)
Binding site324Ca2+ 1 (UniProtKB | ChEBI)
Binding site333Ca2+ 1 (UniProtKB | ChEBI)
Binding site336Ca2+ 1 (UniProtKB | ChEBI)
Binding site337Ca2+ 1 (UniProtKB | ChEBI)
Binding site768Ca2+ 2 (UniProtKB | ChEBI)
Binding site771Ca2+ 2 (UniProtKB | ChEBI)
Binding site794Ca2+ 2 (UniProtKB | ChEBI)
Binding site797Ca2+ 2 (UniProtKB | ChEBI)
Binding site994Ca2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functioncalcium ion binding
Molecular Functioncalcium-activated cation channel activity
Molecular Functionidentical protein binding
Molecular Functionligand-gated calcium channel activity
Biological Processcalcium ion transmembrane transport

Keywords

Protein family/group databases

    • 1.A.4.5.14the transient receptor potential ca2+/cation channel (trp-cc) family

Names & Taxonomy

Protein names

  • Recommended name
    Transient receptor potential cation channel subfamily M member 5

Gene names

    • Name
      trpm5

Organism names

  • Taxonomic identifier
  • Strain
    • Tuebingen
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio

Accessions

  • Primary accession
    S5UH55
  • Secondary accessions
    • A0A0R4IB92
    • A0A8M6Z1J3

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane, intramembrane.

TypeIDPosition(s)Description
Topological domain1-715Cytoplasmic
Transmembrane716-740Helical; Name=1
Topological domain741-751Extracellular
Transmembrane752-771Helical; Name=2
Topological domain772-792Cytoplasmic
Transmembrane793-811Helical; Name=3
Topological domain812-818Extracellular
Transmembrane819-841Helical; Name=4
Topological domain842-850Cytoplasmic
Transmembrane851-880Helical; Name=5
Topological domain881-889Extracellular
Intramembrane890-930Pore-forming
Topological domain931-942Extracellular
Transmembrane943-977Helical; Name=6
Topological domain978-1165Cytoplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis337Altered the voltage sensitivity. Renders TRPM5 voltage-sensitive at high Ca2+ concentration.
Mutagenesis793The antagonist NDNA fails to suppress channel activity.
Mutagenesis836The antagonist NDNA fails to suppress channel activity.
Mutagenesis853The antagonist NDNA fails to suppress channel activity.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004609301-1165Transient receptor potential cation channel subfamily M member 5
Modified residue121Phosphoserine

Keywords

Expression

Gene expression databases

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif904-906Selectivity filter

Domain

Contains two Ca2+-binding sites that are allosterically coupled. The binding site in the intracellular domain modulates the voltage dependence and the accessibility of Ca2+ in the transmembrane domain.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,165
  • Mass (Da)
    132,655
  • Last updated
    2024-10-02 v2
  • Checksum
    835A4EABB94E5561
MVEKSSERFDKQMAGRLGDIDFTGVSRTRGKFVRVTSSTDPAEIYQILTKQWGLAPPHLVVALMGGDEVAQLKPWLRDTLRKGLVKAAQSTGAWILTSGLRFGITKNLGQAVRDHSLASTSPKVRVVAIGIAPWNMIQNRDLLLSAKPDHPATYPTEDLPYGAVYSLDCNHSHFILVDEDPKRPGATGEMRVKMLKHISLQRTGYGGTGSIEIPVLCLLVHGEPRILQKMYKNIQNSIPWLILAGSGGVADILVTLMDRGCWDADIVQELLINTFPDGLHSTEITSWTKLIQRILDHGHLLTVHDPEQDSELDTVILKALVKACKSQSQEAQDFLDELKLAVAWNRVDIAKSEIFSGDVQWSAQDLEEVMMEALVNDKPDFVRLFVDNGVNIKQFLTYGRLQELYCSVSEKNLLHTLLLKKNQERQAQLARKRMSGNPNNELGDRKFRFTFHEVSKVLKDFLDDTCKGFYQKLPAERMGKGRLFHSQKNLPDMDRRCEHPWRDLFLWAILQNRQEMANYFWAMGPEAVAAALVGCKIMKEMAHLATEAESARSMKNAKYEQFAMDLFSECYSNSEDRAYSLLVRKTCCWSKATVLNIATLAEAKCFFAHDGVQALLTKVWWGAMRTDTSISRLVLTFFIPPLVWTSLIKFNPEEQVSKDEGEPFAELDSLETEQALLLTDGDPVAGEGSAETAARSCSATFIRVVLRRWNRFWSAPVTVFMGNVIMYFAFLILFSYVLLLDFRPPPPYGPSAAEIILYFWVFTLVLEEIRQSFFTDEDMSILKKMKLYVEDNWNKCDMVAISLFVVGLSCRMAMSTYEAGRTVLALDFMVFTLRLIHIFAIHKQLGPKIIIVERMIKDVFFFLFFLSVWLIAYGVTTQALLHPNDPRIDWVFRRALYRPYLHIFGQIPLEEIDAAKMPDDNCTTDVQEIILGTLPPCPNIYANWLVILLLVIYLLVTNVLLLNLLIAMFSYTFQVVQENADIFWKFQRYNLIVEYHSRPALAPPFIIISHITQALLSFIKKTENTQDLLERELPSGLDQKLMTWETVQKENYLAKLEHEHRESSGERLRYTSSKVQTLLRMVGGFKDQEKRMATVETEVRYCGEVLSWIAECFHKSTLKCDRDAPKAPRSIAGSSRDQQPQGAKRQQPAGHPAYGTDKKLPFIDH

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2R8QPK7A0A2R8QPK7_DANREtrpm51160

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict1149in Ref. 1; AGS55987

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KF305313
EMBL· GenBank· DDBJ
AGS55987.1
EMBL· GenBank· DDBJ
mRNA
AL928843
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
AL929208
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp