S5R0Y9 · S5R0Y9_9PROT
- ProteinEnterobactin synthase component D
- GenedipB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids222 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.
Catalytic activity
- apo-[aryl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H+ + holo-[aryl-carrier protein]
- apo-[peptidyl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H+ + holo-[peptidyl-carrier protein]
Cofactor
Pathway
Siderophore biosynthesis; enterobactin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 51 | CoA (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 59 | CoA (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 95-96 | CoA (UniProtKB | ChEBI) | ||||
Sequence: SH | ||||||
Binding site | 118 | CoA (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 118 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 119 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 164 | CoA (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 168 | CoA (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | enterobactin synthetase complex | |
Molecular Function | holo-[acyl-carrier-protein] synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | enterobactin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnterobactin synthase component D
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Candidatus Profftella
Accessions
- Primary accessionS5R0Y9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 39-105 | 4'-phosphopantetheinyl transferase N-terminal | ||||
Sequence: EEKKLVINAVSKRKKEFSTGRWLSRKGLKYFGFPETEILTGYLKNPIWPDKINGSISHDNKLCTVVI | ||||||
Domain | 115-199 | 4'-phosphopantetheinyl transferase | ||||
Sequence: IGIDIIYFPKYSNKLKYLESMFVINKNEIDIMDKFGLNIDSSTILFSVKESIIKAISSEINNFIDMRDIKIFYENKKIQFIIQNK |
Sequence similarities
Belongs to the P-Pant transferase superfamily. EntD family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length222
- Mass (Da)25,958
- Last updated2013-10-16 v1
- Checksum504F3211C21F045F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003468 EMBL· GenBank· DDBJ | AGS06837.1 EMBL· GenBank· DDBJ | Genomic DNA |