S5NHA5 · S5NHA5_SALBN
- ProteinNADH-quinone oxidoreductase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids908 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activity
- a quinone + 5 H+(in) + NADH = a quinol + 4 H+(out) + NAD+
CHEBI:132124 + 5 H+ (in)CHEBI:15378+ CHEBI:57945 = CHEBI:24646 + 4 H+ (out)CHEBI:15378+ CHEBI:57540
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 [2Fe-2S] cluster per subunit.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | oxidoreductase complex | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | molybdopterin cofactor binding | |
Molecular Function | NADH dehydrogenase (ubiquinone) activity | |
Molecular Function | quinone binding | |
Biological Process | ATP synthesis coupled electron transport |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-quinone oxidoreductase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionS5NHA5
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-83 | 2Fe-2S ferredoxin-type | ||||
Sequence: MATIHVDGKEYEVNGADNLLQACLSLGLDIPYFCWHPALGSVGACRQCAVKQYQNAEDTRGRLVMSCMTPATDGTFISIDDEE | ||||||
Domain | 83-122 | 4Fe-4S His(Cys)3-ligated-type | ||||
Sequence: EAKQFRESVVEWLMTNHPHDCPVCEEGGNCHLQDMTVMTG | ||||||
Domain | 221-277 | 4Fe-4S Mo/W bis-MGD-type | ||||
Sequence: MQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLKD |
Sequence similarities
Belongs to the complex I 75 kDa subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length908
- Mass (Da)100,029
- Last updated2013-10-16 v1
- Checksum06B3A7D4F9F3B6DF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP006608 EMBL· GenBank· DDBJ | AGR59622.1 EMBL· GenBank· DDBJ | Genomic DNA |