S5MSF0 · S5MSF0_SALBN

Function

Catalytic activity

  • Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
    EC:3.2.1.23 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per monomer.
Na+ (UniProtKB | Rhea| CHEBI:29101 )

Note: Binds 1 sodium ion per monomer.

Features

Showing features for binding site, site, active site.

110251002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site103substrate
Binding site202Na+ (UniProtKB | ChEBI)
Binding site202substrate
Site358Transition state stabilizer
Site392Transition state stabilizer
Binding site417Mg2+ 1 (UniProtKB | ChEBI)
Binding site419Mg2+ 1 (UniProtKB | ChEBI)
Active site462Proton donor
Binding site462Mg2+ 1 (UniProtKB | ChEBI)
Binding site462substrate
Active site538Nucleophile
Binding site538-541substrate
Binding site598Mg2+ 2 (UniProtKB | ChEBI)
Binding site602Na+ (UniProtKB | ChEBI)
Binding site605Na+ (UniProtKB | ChEBI)
Binding site605substrate
Binding site1003substrate

GO annotations

AspectTerm
Cellular Componentbeta-galactosidase complex
Molecular Functionbeta-galactosidase activity
Molecular Functioncarbohydrate binding
Molecular Functionmagnesium ion binding
Biological Processcarbohydrate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Beta-galactosidase
  • EC number
  • Short names
    Beta-gal
  • Alternative names
    • Lactase

Gene names

    • Name
      lacZ
    • ORF names
      A464_322

Organism names

  • Taxonomic identifier
  • Strain
    • N268-08
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella

Accessions

  • Primary accession
    S5MSF0

Proteomes

Subcellular Location

Interaction

Subunit

Homotetramer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain752-1025Beta galactosidase small chain/

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,025
  • Mass (Da)
    117,714
  • Last updated
    2013-10-16 v1
  • Checksum
    C44890C23BE069BC
MNQERDFLSSVLSRRDWENPGVTQLNRLASHPPFYSWRHADDARHDEPSSRRRSLNGQWKFNWFASPEAVPEAWRLHDLQHADTITVPSNWQMYGYDAPIYSNITYPFPVNPPFVPAENPTGCYSLTFIINNDELQDGKTRIIFDGINSAFYLWCNGRWVGYGQDSRLPSEFDLSDFLREGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVYLLHKPSTYISDLHINTHFNDDFSRAVVDAEIRSTGDLHDNLRVTVQLWDGETLIGKRTAPLGSDIIDERGAYHDRTTLRLKVEHPRLWSAETPNLYRAVALLHTADNTLIEAEACDIGFRQVRIENGLLLLNGKRLLIRGVNRHEHHPQQGQVMDYDTMLKDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVNEANIETHGMTPMNRLSDDPIWLPAMSQRVTRMVQRDRNHPSIIIWSLGNESGHGANHDALYRWLKSADVSRPVQYEGGGADTAATDIVCPMYARVDQDQPFPAVPKWSIKKWLSLPGEQRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDADGKPWSAYGGDFGDAPNDRQFCMNGLVFADRTPHPALFEAKHAQQFFQFRLLPGPGRQIEVTSEYLFRHSDNERLRWSLAQNGKPVTGGEVTLDIPPQGHQRITLPEFPQPETAGQLWLTVRVEQPQATAWSQAGHICAWQQWKLAEKLNIQLPPTANTTPQLSVDDHVFTVEVNGKHWQFCRKLGFLIQYRINDEEQLLTPLVDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEASLLQCDADALASAVLITTVHVWQYEGNLLFISRKTYRIDGNGEMQITVDVDVNYGTPPPARIGLNCQLVAVADRVNWLGLGPHESYPDRLSAACFDRWELPLEEMYTPYVFPSENGLRCATRELNYGPHRWRGDFQFNISRFSQQQLMETRHRHLLQPEAGTWLNIDGFHMGVGGDDSWSPSVSAEYVLSAGRYHYQLSWY

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP006608
EMBL· GenBank· DDBJ
AGR57508.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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