S5MQZ8 · S5MQZ8_SALBN
- ProteinFormate-dependent phosphoribosylglycinamide formyltransferase
- GenepurT
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids392 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.
Catalytic activity
- ATP + formate + N1-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H+ + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide + phosphate
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 22-23 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: EL | ||||||
Binding site | 82 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 114 | ATP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 155 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 160-165 | ATP (UniProtKB | ChEBI) | ||||
Sequence: SSGKGQ | ||||||
Binding site | 195-198 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EGVV | ||||||
Binding site | 203 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 267 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 279 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 286 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 355 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 362-363 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: RR |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ligase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphoribosylglycinamide formyltransferase 2 activity | |
Molecular Function | phosphoribosylglycinamide formyltransferase activity | |
Biological Process | 'de novo' IMP biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFormate-dependent phosphoribosylglycinamide formyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella
Accessions
- Primary accessionS5MQZ8
Proteomes
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 119-308 | ATP-grasp | ||||
Sequence: RLAAEELGLPTSEYRFADSEASFHDAVAAMGFPCIVKPVMSSSGKGQSFIRSADQITQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAIDGVHFCAPVGHRQEDGDYRESWQPQQMSELALTRAQDIARTVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFL |
Sequence similarities
Belongs to the PurK/PurT family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length392
- Mass (Da)42,328
- Last updated2013-10-16 v1
- Checksum6C301479457FA9E5
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP006608 EMBL· GenBank· DDBJ | AGR59156.1 EMBL· GenBank· DDBJ | Genomic DNA |