S5DQS6 · PFMT1_OCIBA
- ProteinCation-dependent phenylpropanoid and flavonoid 8-O-methyltransferase 1
- GenePFOMT-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids234 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cation-dependent phenylpropanoid and flavonoid 8-O-methyltransferase involved in the biosynthesis of polymethoxylated flavonoids natural products such as nevadensin and salvigenin, aroma compounds which contribute to the flavor of sweet basil, and exhibit pharmacological activities such as anti-allergic, anti-oxidant, antibacterial, anti-proliferative, and anti-inflammatory effects (PubMed:23747095).
May also act as a flavone 3'-O-methyltransferase (PubMed:23747095).
Catalyzes S-adenosylmethionine-dependent regioselective 8-O-methylation of flavonoids; mediates likely the conversion of pilosin (PIL) to nevadensin (NEV) (PubMed:23747095).
Can also use 3',4',7,8-tetrahydroxyflavone and 3',4',7-trihydroxyflavone as substrates (PubMed:23747095).
Accepts other unnatural O-diphenols including luteolin and 7,8,4'-trihydroxy-flavone, cirsiliol, caffeic acid, 3,4-dihydroxylbenzaldehyde, and 3',4'-dihydroxy-flavone, and, with a lower efficiency, eriodictyol and catechol, as substrates (PubMed:23747095).
May also act as a flavone 3'-O-methyltransferase (PubMed:23747095).
Catalyzes S-adenosylmethionine-dependent regioselective 8-O-methylation of flavonoids; mediates likely the conversion of pilosin (PIL) to nevadensin (NEV) (PubMed:23747095).
Can also use 3',4',7,8-tetrahydroxyflavone and 3',4',7-trihydroxyflavone as substrates (PubMed:23747095).
Accepts other unnatural O-diphenols including luteolin and 7,8,4'-trihydroxy-flavone, cirsiliol, caffeic acid, 3,4-dihydroxylbenzaldehyde, and 3',4'-dihydroxy-flavone, and, with a lower efficiency, eriodictyol and catechol, as substrates (PubMed:23747095).
Catalytic activity
- an 8-hydroxyflavone + S-adenosyl-L-methionine = an 8-methoxyflavone + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
- 4',7,8-trihydroxyflavone + S-adenosyl-L-methionine = 4',7-dihydroxy-8-methoxyflavone + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
- 3',4',7,8-tetrahydroxyflavone + S-adenosyl-L-methionine = 3',4,7-trihydroxy-8-methoxyflavone + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
- 3',4',7-trihydroxyflavone + S-adenosyl-L-methionine = 4',7-dihydroxy-3'-methoxyflavone + H+ + S-adenosyl-L-homocysteineThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 divalent metal cation per subunit.
Biotechnology
Nevadensin is a selective inhibitor of human carboxylesterase 1 (hCE-1), a key enzyme responsible for the hydrolysis of a wide range of endogenous and xenobiotic esters.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
3.98 μM | luteolin | in the presence of S-adenosyl-L-methionine | ||||
8.62 μM | cirsiliol | in the presence of S-adenosyl-L-methionine | ||||
3.31 μM | 7,8,4'-trihydroxy-flavone | in the presence of S-adenosyl-L-methionine | ||||
12.19 μM | S-adenosyl-L-methionine | in the presence of luteolin |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
1.61 nmol/sec/mg | with luteolin as substrate (in the presence of S-adenosyl-L-methionine) | ||||
1.22 nmol/sec/mg | with cirsiliol as substrate (in the presence of S-adenosyl-L-methionine) | ||||
4.3 nmol/sec/mg | with 7,8,4'-trihydroxy-flavone as substrate (in the presence of S-adenosyl-L-methionine) | ||||
1.94 nmol/sec/mg | with S-adenosyl-L-methionine as substrate (in the presence of luteolin) |
kcat is 45.39 sec-1 with luteolin as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:23747095).
kcat is 34.30 sec-1 with cirsiliol as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:23747095).
kcat is 121.1 sec-1 with 7,8,4'-trihydroxy-flavone as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:23747095).
kcat is 54.77 sec-1 with S-adenosyl-L-methionine as substrate (in the presence of luteolin) (PubMed:23747095).
kcat is 34.30 sec-1 with cirsiliol as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:23747095).
kcat is 121.1 sec-1 with 7,8,4'-trihydroxy-flavone as substrate (in the presence of S-adenosyl-L-methionine) (PubMed:23747095).
kcat is 54.77 sec-1 with S-adenosyl-L-methionine as substrate (in the presence of luteolin) (PubMed:23747095).
Pathway
Flavonoid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 52 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 74 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 76-77 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: GV | ||||||
Binding site | 82 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 100 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 129 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 152 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 154 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 178 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 179 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | caffeoyl-CoA O-methyltransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | lignin biosynthetic process | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCation-dependent phenylpropanoid and flavonoid 8-O-methyltransferase 1
- EC number
- Short namesObPFOMT-1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Lamiaceae > Nepetoideae > Ocimeae > Ociminae > Ocimum
Accessions
- Primary accessionS5DQS6
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000456920 | 1-234 | Cation-dependent phenylpropanoid and flavonoid 8-O-methyltransferase 1 | |||
Sequence: MEGKDGSIDKGLLQSTELYKYILDTSVYPREEECLKELRALTWTHPRAVMGTAPETGQFMALLLKTINAKKTLEIGVFTGYSLLLTALAIPHDGKITAIDINRDTYEIGLPIIEKAGVKHKIDFIESKALPALDHLLKDGENKESFDFVFVDADKVNYANYHERVLELLRPGGIVVYDNTLWGGTVAMAPDLVAESKLQYRNAAVEFNNFIAADSRVQISQLPVGDGITVCRRK |
Expression
Tissue specificity
Expressed in leaves and trichomes.
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length234
- Mass (Da)26,029
- Last updated2013-10-16 v1
- Checksum07197453A2A9ABD6