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S0E2Y4 · GA1_GIBF5

Function

function

GA4 desaturase; part of the gene cluster that mediates the biosynthesis of gibberellins (GAs), diterpenoids that may provide a selective advantage during infection of the preferred host plant, rice (PubMed:10347043, PubMed:12750377, PubMed:15925394, PubMed:23825955, PubMed:9917370).
Gibberellins (GAs) are diterpenoids and are synthesized via the mevalonate pathway (PubMed:12750377).
Biosynthesis of the major metabolite GA3 (gibberellic acid) from geranylgeranyl diphosphate (GGPP) requires 13 steps (PubMed:12750377).
The GGPP produced by the geranylgeranyl diphosphate synthase GGS2 is converted to ent-kaurene via ent-copalyldiphosphate in a two-step cyclization reaction performed by the bifunctional ent-copalyl diphosphate synthase/ent-kaurene synthase enzyme (CPS/KS) (PubMed:10803977, PubMed:12750377, PubMed:9745028).
Ent-Kaurene is metabolized to GAs by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenases (PubMed:12750377, PubMed:9917370).
Cytochrome P450 monooxygenase P450-4 is an ent-kaurene oxidase that catalyzes the three oxidation steps between ent-kaurene and ent-kaurenoic acid (PubMed:11472927).
The highly multifunctional cytochrome P450 monooxygenase P450-1 then catalyzes four steps involving oxidation at two carbon atoms, in the main pathway from ent-kaurenoic acid to GA14 via GA12-aldehyde as well as producing kaurenolides and fujenoic acids as by-products (PubMed:11320210).
The cytochrome P450 monooxygenase P450-2 then converts GA14 to GA4 by removal of C-20 (PubMed:11943776).
GA4 is further converted to GA7 by the GA4 desaturase DES via 1,2-desaturation before cytochrome P450 monooxygenase P450-3, a 13-hydroxylase, hydroxylates GA7 to GA3, the final product of the GA-biosynthetic pathway (PubMed:12750377).

Pathway

Plant hormone biosynthesis; gibberellin biosynthesis.

GO annotations

AspectTerm
Molecular Functionoxidoreductase activity
Biological Processgibberellin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Gibberellin cluster GA4 desaturase
  • EC number
  • Short names
    DES

Gene names

    • Name
      DES
    • ORF names
      FFUJ_14331

Organism names

Accessions

  • Primary accession
    S0E2Y4

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Impairs the production of GA3 and of its immediate precursor GA7, but accumulates GA4 and GA1 (PubMed:12750377).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004420401-342Gibberellin cluster GA4 desaturase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region127-183Disordered
Compositional bias160-175Basic and acidic residues

Sequence similarities

Belongs to the asaB hydroxylase/desaturase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    342
  • Mass (Da)
    37,863
  • Last updated
    2013-09-18 v1
  • MD5 Checksum
    F729C2B5D052F381E89FE876AE53020A
MPHKDNLLESPVGKSVTATIAYHSGPALPTSPIAGVTTLQDCTQQAVAVTDIRPSVSSFTLDGNGFQVVKHTSAVGSPPYDHSSWTDPVVRKEVYDPEIIELAKSLTGAKKVMILLASSRNVPFKEPELAPPYPMPGKSSSGSKEREAIPANELPTTRAKGFQKGEEEGPVRKPHKDWGPSGAWNTLRNWSQELIDEAGDIIKAGDEAAKLPGGRAKNYQGRRWALYTTWRPLKTVKRDPMAYVDYWTADEEDGVSFWRNPPGVHGTFESDVLLTKANPKHKWYWISDQTPDEVLLMKIMDTESEKDGSEIAGGVHHCSFHLPGTEKEEVRESIETKFIAFW

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias160-175Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HF679027
EMBL· GenBank· DDBJ
CCT69176.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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