S0DUX5 · FUB7_GIBF5

Function

function

Sulfhydrylase; part of the gene cluster that mediates the biosynthesis of fusaric acid, a mycotoxin with low to moderate toxicity to animals and humans, but with high phytotoxic properties (PubMed:26662839).
L-aspartate is suggested as fusaric acid amino acid precursor that is activated and further processed to O-acetyl-L-homoserine by cluster enzymes aspartate kinase FUB3 and homoserine O-acetyltransferase FUB5, as well as enzymes of the primary metabolism (PubMed:26662839).
The polyketide synthase (PKS) FUB1 generates the triketide trans-2-hexenal which is presumptively released by the hydrolase FUB4 and linked to the NRPS-bound amino acid precursor by NAD(P)-dependent dehydrogenase FUB6 (PubMed:26662839).
FUB1, FUB4, and the non-canonical NRPS Fub8 may form an enzyme complex (PubMed:26662839).
Further processing of the NRPS-bound intermediate might be carried out by FUB6 and the O-acetylhomoserine FUB7, enabling a spontaneous electrocyclization to close the carbon backbone of fusaric acid (PubMed:26662839).
Dihydrofusaric acid is likely to be released via reduction by the thioester reductase (TR) domain of FUB8 whereupon the final oxidation to fusaric acid may (also) be performed by the FMN-dependent dehydrogenase FUB9 (PubMed:26662839).

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Biotechnology

Fusaric acid is phytotoxic to plants such as cotton and banana (PubMed:20955724, PubMed:23922960).
It has been shown to induce programmed cell death in plants (PubMed:16868776, PubMed:23838885).
In addition to a mild toxicity to animals, fusaric acid exhibits acanthamoebicidal, antioomycete, and antimycobacterial activities (PubMed:17927749, PubMed:21811925, PubMed:22864988).

Pathway

Mycotoxin biosynthesis.

GO annotations

AspectTerm
Molecular Functionpyridoxal phosphate binding
Molecular Functiontransferase activity, transferring alkyl or aryl (other than methyl) groups
Biological Processtranssulfuration

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sulfhydrylase FUB7
  • EC number
  • Alternative names
    • Fusaric acid biosynthesis protein 7

Gene names

    • Name
      FUB7
    • ORF names
      FFUJ_02114

Organism names

Accessions

  • Primary accession
    S0DUX5

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Impairs the production of fusaric acid, but leads to the accumulation of trans-2-hexenal (PubMed:26662839).

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00004373361-433Sulfhydrylase FUB7
Modified residue211N6-(pyridoxal phosphate)lysine

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the trans-sulfuration enzymes family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    433
  • Mass (Da)
    47,128
  • Last updated
    2013-09-18 v1
  • Checksum
    CC9DE8C5FD899DE3
MAEQVFQNFETLQLHAGYTPDPHTRSTAVPIYATSSYTFNDSAHGARLFGLKELGNIYSRLMNPTVDVFEKRIAALEGGIAAAATSSGQAAQFLTIATLAKAGDNIVASSHLYGGTYNQLNVLLPRFGIKTKFVRSGKLEDYAAAIDDQTRAIYVESMSNPDYVVPDFEGIAKIAHEHGIPLVVDNTLGAGGYYIRPIEHGADIVVHSATKWIGGHGTTIGGVIVDSGRFNWNKHSDRFPEMVEPSPSYHGLKYWEAFGPATFITRIRVEMLRDIGACLSPFSAQQLLLGIETLGLRAERHAQNTEKLSKYFESSPNVSWVLWPGSESHPTYSQAKKYLTRGFGAMLSIGVKGDASAGSKVVDGLKLVSNLANVGDAKSLAIHPWSTTHEQLSEDERLASGVTEDMIRISVGIEHVDDIIADFEQSFQKAYGS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
HF679025
EMBL· GenBank· DDBJ
CCT65192.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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