S0AWD4 · S0AWD4_ENTHI

  • Protein
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    4/5

Function

function

Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Non-allosteric.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site80diphosphate (UniProtKB | ChEBI)
Binding site174Mg2+ (UniProtKB | ChEBI); catalytic
Site175Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site201Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site202-204substrate
Active site204Proton acceptor
Binding site241-242substrate; ligand shared between dimeric partners
Binding site249-251substrate
Binding site310substrate
Binding site420-423substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase
  • EC number
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-dependent phosphofructokinase
      (PPi-PFK
      )
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • HM-1:IMSS
  • Taxonomic lineage
    Eukaryota > Amoebozoa > Evosea > Archamoebae > Mastigamoebida > Entamoebidae > Entamoeba

Accessions

  • Primary accession
    S0AWD4

Organism-specific databases

Subcellular Location

Keywords

Interaction

Subunit

Homodimer or monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain72-328Phosphofructokinase

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    546
  • Mass (Da)
    60,162
  • Last updated
    2013-09-18 v1
  • Checksum
    562DB85D8F7A8360
MSLSALHKYRLQYKPVLPKHIADIDNITIEEGAKTQSAVNQKELSELFKHTYGLPICNIVAGKNADIHRVIRCGFILSGGPAAGGHNVVAGLFDGLMKGNKENKLYGFRCGAGGILSNDYIEITAELVDKHRNTGGFDLVGSGRTKIETEEQFATAFEHITALKLNAMVVVGGDDSNTNAALLAEYFAAHGSDCVFVGVPKTIDGDLKNQYIETSFGFDTACKTYSELIGNIQRDAISSRKYWHFIKVMGRSASHIALEAALETQPTYCIISEEVEDKKMTVSQIASEIADIVIERHKKGLNFGVVLIPEGLVEFIPEVKALIKELNNLLAHKKEEYSKITEFSAQKAFVCENISESCAATFKNLPDNIAKQLLLDRDPHGNVNVSAIETESFVSGIVKAEIVKRGIKVPFTPVHHFFGYEGRCAFPSNFDSTYCYALGYTAFILLALKKTGQICCISGLQKPAEEWICGGVPLTIMMNMEQRNGEMKPVIKKALVEIEGKPFKFYQSKRAQWASAEDFVFPGAIQYFGPSEVCDQPTKTLLLEQN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK421127
EMBL· GenBank· DDBJ
BAN39695.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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