R9QMR4 · BPHL1_PINCO
- Protein(-)-beta-phellandrene synthase 1, chloroplastic
- GeneTPS-(-)Bphell1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids621 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Monoterpene synthase (TPS) involved in the biosynthesis of monoterpene natural products included in conifer oleoresin secretions and volatile emissions; these compounds contribute to biotic and abiotic stress defense against herbivores and pathogens (PubMed:23679205).
Catalyzes the conversion of (2E)-geranyl diphosphate (GPP) to --beta-phellandrene and, to a lower extent, to --alpha-phellandrene (PubMed:23679205).
Catalyzes the conversion of (2E)-geranyl diphosphate (GPP) to --beta-phellandrene and, to a lower extent, to --alpha-phellandrene (PubMed:23679205).
Catalytic activity
- (2E)-geranyl diphosphate = --beta-phellandrene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Pathway
Terpene metabolism; oleoresin biosynthesis.
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | green leaf volatile biosynthetic process | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(-)-beta-phellandrene synthase 1, chloroplastic
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Pinopsida > Pinidae > Conifers I > Pinales > Pinaceae > Pinus > Pinus subgen. Pinus
Accessions
- Primary accessionR9QMR4
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-49 | Chloroplast | ||||
Sequence: MALALVSVAPLVSMRRSLFSSPYELKSIDKTIPNLVMCRKRMSGTPSIR | ||||||
Chain | PRO_0000455015 | 50-621 | --beta-phellandrene synthase 1, chloroplastic | |||
Sequence: VSSTTSASNDDGVRRRVGDYRYNHWDDDLIDSLATSYEAPSYLERADTLVEAIKDRFNSMGVDDGERMSPLTDLYQRLWMVDSVERLGIDRHFQNEIKSALDYVFSYWKEKGIGRGRQSAVTDLNSTALGLRTLRLHGYPVSSDVLENFKDHNGQFTCSGIQTEGEIRGVLNLFRASLIAFPGEKVMEEAEIFSTMYLKHALQKIAVSSLSQEIEYLLEYGWHTNPPRLEARMYMEVFPQDTIYEQKLVELAKVEFNIFHSLQKRELQSLTRWWKHYGFPQLSFTRHIHVEYYTFGSCIATDPKQSAFRLCFAKMSYFVTVLDDIYDTYGTMEELELFTAAIKRWDPSVVDCLPEYMKGVYMAVYDTVNEMAKEAEKVQGRDTLNYVRQAWELYIDAYMPEAKWISSGYLPTFQEYLDNSKISFGTRITILQPILTLGEPLPHEILQEIDFPAKFNDLISVILRLKGDTRCYKADRARGEEASSVSCYMKDNAGITEEDAVHCINDMVNNLLKELNWELLKPDSNVPISCRKAAFDICRIFHHGYKYRDGYGDATIEVKNLVKRTVLEPVPL |
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 372-376 | DDXXD motif | ||||
Sequence: DDIYD |
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsd subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length621
- Mass (Da)71,452
- Last updated2013-09-18 v1
- Checksum2461B38A51B71A03