R8BEE4 · R8BEE4_PHAM7

Function

function

Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).
Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 2 Fe2+ ions per subunit.

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis.
Protein modification; eIF5A hypusination.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site963Fe cation 1 (UniProtKB | ChEBI)
Binding site964Fe cation 1 (UniProtKB | ChEBI)
Binding site996Fe cation 1 (UniProtKB | ChEBI)
Binding site997Fe cation 1 (UniProtKB | ChEBI)
Binding site1128Fe cation 2 (UniProtKB | ChEBI)
Binding site1129Fe cation 2 (UniProtKB | ChEBI)
Binding site1161Fe cation 2 (UniProtKB | ChEBI)
Binding site1162Fe cation 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Cellular Componentnucleus
Molecular Functiondeoxyhypusine monooxygenase activity
Molecular Functionmetal ion binding
Molecular Functionphosphatidylethanolamine N-methyltransferase activity
Biological Processmethylation
Biological Processpeptidyl-lysine modification to peptidyl-hypusine
Biological Processphosphatidylcholine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Multifunctional fusion protein

Including 2 domains:

  • Recommended name
    Deoxyhypusine hydroxylase
  • EC number
  • Short names
    DOHH
  • Alternative names
    • Deoxyhypusine dioxygenase
    • Deoxyhypusine monooxygenase
  • Recommended name
    Phosphatidylethanolamine N-methyltransferase
  • EC number
  • Short names
    PE methyltransferase
    ; PEAMT
    ; PEMT

Gene names

    • Name
      LIA1
    • ORF names
      UCRPA7_6839

Organism names

Accessions

  • Primary accession
    R8BEE4

Proteomes

Subcellular Location

Cytoplasm
Nucleus
Endomembrane system
; Multi-pass membrane protein
Endoplasmic reticulum membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane37-54Helical
Transmembrane61-81Helical
Transmembrane147-167Helical
Transmembrane174-195Helical
Transmembrane332-350Helical
Transmembrane356-379Helical
Transmembrane496-529Helical

Keywords

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region281-311Disordered
Compositional bias295-311Polar residues
Region1044-1070Disordered

Sequence similarities

Belongs to the deoxyhypusine hydroxylase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,223
  • Mass (Da)
    137,257
  • Last updated
    2013-07-24 v1
  • Checksum
    8167885D5A326FE7
MKTYGKTPDGTVFIVPTTHDMVSQLLDPRQPKNLSDALVLAILALHILAAYLLPDGLKKPVFAAIFLWWRASYNIGIGVLLRVQSNHKLLVKWAKKWRLFENPASGNQPRPWLYKLLKNELEAKIPEDYEFEQAPIEYNTWLVFRRVVDLILMCDFVSYCLFAIVCAHQPESEGLSLFLGRWVVGIALFGFNLWVKLDAHRVVKDYAWYWGDFFYLIEQELTFDGVFEMAPHPMYSIGYAGYYGISAMAASYEVLFISIAAHLAQMIFLVVVENPHIEKTYNPPPPRVRSDGGRESFGSQESETKPPNQEVPSQIHNLLGFKNLDLFRTTDYAVILLVFYTVCMTVFTPTTPIYQALFVLHALFWRLWYSVGLGIMLNLQSKEKMFTRHFVKYGESAGEAWRQWKGMYHLSLTMCHASFIAACWKMYSLPDDWSYGWVMLKHVVGFGLIALQIWTAVSIYDELGEFGWFFGDFFFDHKVKLTYKSIYRFLNNPERVMGLAGLWGAALITWSRAIFLLAIVSQILTLGFIELVEKPHMQKVYGRRNIRDEAGLTKFIKKSLPAPVKGWQSSVDKVLDETKHFVDDVVEAARPLFAAGVSTIVRDTTQLFNKYPARLTITRLSPDLAGYDPKHYSLSVEGTPSLSGINERATGKESLTARVPKDVKTAVFEYGAPIRVKWTAPANHSKKDWIGLYMVTDNRSRDVTEVSSLGRWAPTVPGGYDMASDKGVLVSEKPVPQSGDSDVKLVQGEMIFEGDRLWWTQGVFEFRLHHDGHHNVMSISEPFEIKISKFDEEDVQADSGGLYQQAVESALLPIVQNCLDRDPDIAPNTVDEPYGSHVERDSKYAKRVVYAIHHMFGIEFAPAVVPADGNVRKLAWRICNAKQVLAPYSMSPSLDVVDASSAVDPTILSLRKSLCSEDSPLPVRFRALFSLKHLATNSTGQDALAAIDAIAAAFSSPSALLKHELAYCLGQTGNMASTPYLRQVLEDLEEDTMCRHEAAEALGALGDAQNLDILKQYRDREGEDVAVKETCEIAIDRLEWENSEQRKQEKLRQSDFASVDPAPPMPESKRTVDDLGKALMDTTLPLFLRYRAMFALRDLASPPDLPTAVPAVLALAKGFADASALFRHEIAFVFGQLSHPASIPALTAALSNLEEASMVRHEAAEALGSLGEEDGVEDVLKRFLHDKERVVRESVIVALDIAEYEKGGEAEYALIPETGSTVA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias295-311Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KB933264
EMBL· GenBank· DDBJ
EON97668.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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