R7WT97 · R7WT97_9NOCA

  • Protein
    Arginine biosynthesis bifunctional protein ArgJ
  • Gene
    argJ
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N2-acetylornithine and glutamate.

Miscellaneous

Some bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N2-acetyl-L-ornithine (cyclic): step 1/1.
Amino-acid biosynthesis; L-arginine biosynthesis; N2-acetyl-L-ornithine from L-glutamate: step 1/4.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site141Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Site142Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole
Binding site180substrate
Binding site203substrate
Site213-214Cleavage; by autolysis
Active site214Nucleophile
Binding site214substrate
Binding site294substrate
Binding site413substrate
Binding site418substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionacetyl-CoA:L-glutamate N-acetyltransferase activity
Molecular Functionglutamate N-acetyltransferase activity
Molecular Functionmethione N-acyltransferase activity
Biological Processarginine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Including 2 domains:

  • Recommended name
    Glutamate N-acetyltransferase
  • EC number
  • Alternative names
    • Ornithine acetyltransferase
      (OATase
      )
    • Ornithine transacetylase
  • Recommended name
    Amino-acid acetyltransferase
  • EC number
  • Alternative names
    • N-acetylglutamate synthase
      (AGSase
      )

Gene names

    • Name
      argJ
    • ORF names
      Rrhod_0028

Organism names

  • Taxonomic identifier
  • Strain
    • LMG 5362
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Nocardiaceae > Rhodococcus

Accessions

  • Primary accession
    R7WT97

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_50234170271-213Arginine biosynthesis bifunctional protein ArgJ alpha chain
ChainPRO_5023417028214-418Arginine biosynthesis bifunctional protein ArgJ beta chain

Keywords

Interaction

Subunit

Heterotetramer of two alpha and two beta chains.

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues
Region1-24Disordered

Sequence similarities

Belongs to the ArgJ family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    418
  • Mass (Da)
    42,246
  • Last updated
    2013-07-24 v1
  • Checksum
    F281FD8E9147F250
MTTPPSSSTPSTPLHATPDADGRLVRNQGVTGPAGFRAAGIAAGIKASGRPDLALVVNEGPELAAAGVFTTNKVKAAPVLWSQQVLASASLRAVILNSGGANACTGAPGFQDAHRTAEHLAAELSNWGTETGAIEVAVCSTGLIGDRLPMDTVLAGVTEIVHELAGGISGGTDAAHAIMTTDTVPKQAALHHVGKWNVGGMAKGAGMLAPSLATMLCVLTTDAAATPEQLDHALRAATRLTFDRLDVDGATSTNDTVLLLASGASNVAPSQDELDAAVLAVCDDLAAQLMADAEGVTKRVTITVRGARTEDEALVGARAVARDSLVKTALFGSDPNWGRVLAAIGAAPVELDPDRISVAFDGNAVCIDGVGAPGARDVDLSGEDITVVVDLAVGDGEASVRTTDLSHAYVEENSAYSS

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-15Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
APMY01000003
EMBL· GenBank· DDBJ
EOM78492.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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