R4Z5D7 · R4Z5D7_9ACTN
- ProteinDNA polymerase IV
- GenedinB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids404 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.
Catalytic activity
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
Cofactor
Note: Binds 2 magnesium ions per subunit.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 13 | Mg2+ (UniProtKB | ChEBI) | |||
Site | 18 | Substrate discrimination | |||
Binding site | 103 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 104 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | damaged DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA-templated DNA replication | |
Biological Process | error-prone translesion synthesis | |
Biological Process | SOS response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase IV
- EC number
- Short namesPol IV
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Acidimicrobiia > Acidimicrobiales > Microthrixaceae > Candidatus Microthrix
Accessions
- Primary accessionR4Z5D7
Proteomes
Subcellular Location
Interaction
Structure
Sequence
- Sequence statusComplete
- Length404
- Mass (Da)43,635
- Last updated2013-07-24 v1
- MD5 Checksum712442ADD7B20D8D88145C44FC41653B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CANL01000080 EMBL· GenBank· DDBJ | CCM65905.1 EMBL· GenBank· DDBJ | Genomic DNA |