R4Z231 · R4Z231_9ACTN

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site51L-glutamine (UniProtKB | ChEBI)
Binding site224L-glutamine (UniProtKB | ChEBI)
Binding site226L-glutamine (UniProtKB | ChEBI)
Active site251Nucleophile
Binding site252L-glutamine (UniProtKB | ChEBI)
Binding site255L-glutamine (UniProtKB | ChEBI)
Binding site293L-glutamine (UniProtKB | ChEBI)
Binding site296L-glutamine (UniProtKB | ChEBI)
Active site334
Active site336

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      pyrAA
    • Synonyms
      carA
    • ORF names
      BN381_50141

Organism names

Accessions

  • Primary accession
    R4Z231

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-175CPSase
Domain4-137Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    37,838
  • Last updated
    2013-07-24 v1
  • Checksum
    C6C392AA94DCC8F7
MSVTEAALVLSDGSLFEGEAIGARPEGGVSTGEVVFNTVLAGYQEVLSDPSYAGQIVTFTYPHIGNYGSAGADDESPTPRCAGVVVRDLARRHSSWRAEASLEDWLKRHGIAGIAGVDTRRLTRHLRNHGAVPGAFGTASEAEVRAAAAGALGTDDTDLTRVVTTSKPYVLAGGPRRVVAVDYGIKRSLFAHLQPIAGVTVVPAGFSADDILALEPDGVLLSNGPGDPAAVTHAPAMIAELLGRVPVFGVCMGHQLLATALGASTYKLPFGHHGGNHPVQDQLTNSIEITSQNHNYCVNPKGIQGVELTHVNLNDQTLEGFIGTDVAALGIQYHPEAGPGPHDSHYLFDRFAEMMDRKAT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CANL01000045
EMBL· GenBank· DDBJ
CCM64999.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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