R4K7G3 · R4K7G3_CLOPA
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1142 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 119 | ATP (UniProtKB | ChEBI) | |||
Binding site | 203 | ATP (UniProtKB | ChEBI) | |||
Binding site | 238 | ATP (UniProtKB | ChEBI) | |||
Active site | 295 | ||||
Binding site | 537 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 609 | substrate | |||
Binding site | 706 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 735 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 737 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 870 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionR4K7G3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 706 | N6-carboxylysine | |||
Modified residue | 1108 | N6-biotinyllysine | |||
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 3-456 | Biotin carboxylation | |||
Domain | 123-320 | ATP-grasp | |||
Domain | 528-796 | Pyruvate carboxyltransferase | |||
Domain | 1067-1142 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,142
- Mass (Da)127,343
- Last updated2013-07-24 v1
- ChecksumC16AD5572E784BD0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP003261 EMBL· GenBank· DDBJ | AGK99117.1 EMBL· GenBank· DDBJ | Genomic DNA |