R0A5G7 · R0A5G7_9FIRM
- ProteinMethionine--tRNA ligase
- GenemetG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids683 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.
Catalytic activity
- tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 150 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 153 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 167 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 170 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | methionine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Biological Process | methionyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethionine--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Lachnospirales > Lachnospiraceae > Enterocloster
Accessions
- Primary accessionR0A5G7
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for motif, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 35-45 | 'HIGH' region | |||
Motif | 322-326 | 'KMSKS' region | |||
Region | 548-569 | Disordered | |||
Domain | 582-683 | TRNA-binding | |||
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length683
- Mass (Da)77,050
- Last updated2013-06-26 v1
- Checksum1210C70D338F9B03
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AGYH01000016 EMBL· GenBank· DDBJ | ENZ47321.1 EMBL· GenBank· DDBJ | Genomic DNA |