Q9ZW95 · C7352_ARATH
- ProteinCytokinin hydroxylase
- GeneCYP735A2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids512 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Cytokinin hydroxylase that catalyzes the biosynthesis of trans-zeatin via the isopentenyladenine riboside 5'-monophosphate (iPRMP)-dependent pathway. Can use isopentenyladenosine-5'-monophosphate, isopentenyladenosine-5'-diphosphate and isopentenyladenosine-5'-triphosphate as substrate.
Catalytic activity
- N6-(dimethylallyl)adenosine 5'-phosphate + NADPH + O2 + H+ = 9-ribosyl-trans-zeatin 5'-phosphate + NADP+ + H2O
- N6-(dimethylallyl)adenosine 5'-diphosphate + NADPH + O2 + H+ = 9-ribosyl-trans-zeatin 5'-diphosphate + NADP+ + H2O
- N6-(dimethylallyl)adenosine 5'-triphosphate + NADPH + O2 + H+ = 9-ribosyl-trans-zeatin 5'-triphosphate + NADP+ + H2O
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.39 μM | isopentenyladenine riboside 5'-monophosphate | |||||
0.76 μM | isopentenyladenine riboside 5'-diphosphate | |||||
4.9 μM | isopentenyladenine riboside 5'-triphosphate |
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 458 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Cellular Component | plasmodesma | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | trans-zeatin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytokinin hydroxylase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9ZW95
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 2-22 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000411209 | 1-512 | Cytokinin hydroxylase | ||
Proteomic databases
Expression
Tissue specificity
Specifically expressed in roots.
Induction
By trans-zeatin, cis-zeatin and isopentenyladenine in roots. Down-regulated by auxin and abscisic acid in roots.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length512
- Mass (Da)58,463
- Last updated1999-05-01 v1
- Checksum038844E878935BC7
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8AWC5 | A0A1P8AWC5_ARATH | CYP735A2 | 375 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC004146 EMBL· GenBank· DDBJ | AAD10659.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE34597.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT011622 EMBL· GenBank· DDBJ | AAS47628.1 EMBL· GenBank· DDBJ | mRNA | ||
BT012626 EMBL· GenBank· DDBJ | AAT06445.1 EMBL· GenBank· DDBJ | mRNA |