Q9ZW85 · LEUD3_ARATH
- Protein3-isopropylmalate dehydratase small subunit 1
- GeneSSU1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids251 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (PubMed:19597944).
Plays an essential role in leucine biosynthesis (PubMed:19597944, PubMed:20663849, PubMed:24608865, PubMed:32612621).
Functions in both the biosynthesis of leucine, and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:24608865).
Plays an essential role in female gametophyte development (PubMed:19597944, PubMed:20663849, PubMed:24608865).
Plays an essential role in leucine biosynthesis (PubMed:19597944, PubMed:20663849, PubMed:24608865, PubMed:32612621).
Functions in both the biosynthesis of leucine, and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:24608865).
Plays an essential role in female gametophyte development (PubMed:19597944, PubMed:20663849, PubMed:24608865).
Miscellaneous
Plants silencing SSU1 exhibit stunted growth, narrow pale leaf blades with green vasculature, abnormal leaf adaxial-abaxial patterning, and abnormal flower morphology.
Catalytic activity
- (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast stroma | |
Cellular Component | plastid | |
Molecular Function | 3-isopropylmalate dehydratase activity | |
Biological Process | chloroplast organization | |
Biological Process | gametophyte development | |
Biological Process | glucosinolate biosynthetic process | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-isopropylmalate dehydratase small subunit 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9ZW85
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Embryonic lethality when homozygous.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-59 | Chloroplast | ||||
Sequence: MAASLQSANPTLSRTLASPNKPSSFATFRSPFLRFNSTSVASNFKPLVSREASSSFVTR | ||||||
Chain | PRO_0000425812 | 60-251 | 3-isopropylmalate dehydratase small subunit 1 | |||
Sequence: SAAEPQERKTFHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASYKERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFRNSVATGEVYPLDSEVRVCDECTTGDVATVELREGDSILINHTTGKEYKLKPIGDAGPVIDAGGIFAYARKAGMIPSAAA |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at low levels in roots, root tips, at the basis of the hypocotyls, and in emerging leaves (PubMed:24608865).
In young seedlings, expressed in cotyledon epidermal cells (PubMed:32612621).
In hypocotyls, expressed in peripheral cells (PubMed:32612621).
In seedling roots, expressed in the epidermis, including root hairs, and throughout the cortex (PubMed:32612621).
In rosette leaves, expressed in the upper and lower epidermis (PubMed:32612621).
In roots of adult plants, expressed in the root tips and cortex of the mature root enclosing the stele (PubMed:32612621).
In flowering stalks, expressed in the epidermis (PubMed:32612621).
Expressed in the carpel epidermis (PubMed:32612621).
In young seedlings, expressed in cotyledon epidermal cells (PubMed:32612621).
In hypocotyls, expressed in peripheral cells (PubMed:32612621).
In seedling roots, expressed in the epidermis, including root hairs, and throughout the cortex (PubMed:32612621).
In rosette leaves, expressed in the upper and lower epidermis (PubMed:32612621).
In roots of adult plants, expressed in the root tips and cortex of the mature root enclosing the stele (PubMed:32612621).
In flowering stalks, expressed in the epidermis (PubMed:32612621).
Expressed in the carpel epidermis (PubMed:32612621).
Gene expression databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.
Q9ZW85-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length251
- Mass (Da)26,790
- Last updated1999-05-01 v1
- Checksum62EBB1B6059ED272
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
F4IQ61 | F4IQ61_ARATH | IPMI | 222 |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC004450 EMBL· GenBank· DDBJ | AAC64298.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC006224 EMBL· GenBank· DDBJ | AAM15163.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC10207.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY035158 EMBL· GenBank· DDBJ | AAK59662.1 EMBL· GenBank· DDBJ | mRNA | ||
AY063029 EMBL· GenBank· DDBJ | AAL34203.1 EMBL· GenBank· DDBJ | mRNA | ||
AY087084 EMBL· GenBank· DDBJ | AAM64645.1 EMBL· GenBank· DDBJ | mRNA |