Q9ZW85 · LEUD3_ARATH

Function

function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (PubMed:19597944).
Plays an essential role in leucine biosynthesis (PubMed:19597944, PubMed:20663849, PubMed:24608865, PubMed:32612621).
Functions in both the biosynthesis of leucine, and in the methionine chain elongation pathway of aliphatic glucosinolate formation (PubMed:24608865).
Plays an essential role in female gametophyte development (PubMed:19597944, PubMed:20663849, PubMed:24608865).

Miscellaneous

Plants silencing SSU1 exhibit stunted growth, narrow pale leaf blades with green vasculature, abnormal leaf adaxial-abaxial patterning, and abnormal flower morphology.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast stroma
Cellular Componentplastid
Molecular Function3-isopropylmalate dehydratase activity
Biological Processchloroplast organization
Biological Processgametophyte development
Biological Processglucosinolate biosynthetic process
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydratase small subunit 1
  • EC number
  • Alternative names
    • AtLEUD3
    • Isopropylmalate isomerase small subunit 1
      (IPMI SSU1
      )

Gene names

    • Name
      SSU1
    • Synonyms
      LEUD3
    • ORF names
      MFL8.15
    • Ordered locus names
      At2g43090

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q9ZW85

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Embryonic lethality when homozygous.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-59Chloroplast
ChainPRO_000042581260-2513-isopropylmalate dehydratase small subunit 1

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed at low levels in roots, root tips, at the basis of the hypocotyls, and in emerging leaves (PubMed:24608865).
In young seedlings, expressed in cotyledon epidermal cells (PubMed:32612621).
In hypocotyls, expressed in peripheral cells (PubMed:32612621).
In seedling roots, expressed in the epidermis, including root hairs, and throughout the cortex (PubMed:32612621).
In rosette leaves, expressed in the upper and lower epidermis (PubMed:32612621).
In roots of adult plants, expressed in the root tips and cortex of the mature root enclosing the stele (PubMed:32612621).
In flowering stalks, expressed in the epidermis (PubMed:32612621).
Expressed in the carpel epidermis (PubMed:32612621).

Gene expression databases

Interaction

Subunit

Heterodimer of the large LEUC/IIL1 subunit and the small LEUD (SSU1, SSU2 or SSU3) subunits.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the LeuD family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 1 isoforms produced by Alternative splicing. A number of isoforms are produced. According to EST sequences.

Q9ZW85-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    251
  • Mass (Da)
    26,790
  • Last updated
    1999-05-01 v1
  • Checksum
    62EBB1B6059ED272
MAASLQSANPTLSRTLASPNKPSSFATFRSPFLRFNSTSVASNFKPLVSREASSSFVTRSAAEPQERKTFHGLCYVVGDNIDTDQIIPAEFLTLVPSNPEEYEKLGSYALVGLPASYKERFVQPGEMKTKYSIIIGGENFGCGSSREHAPVCLGAAGAKAVVAQSYARIFFRNSVATGEVYPLDSEVRVCDECTTGDVATVELREGDSILINHTTGKEYKLKPIGDAGPVIDAGGIFAYARKAGMIPSAAA

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
F4IQ61F4IQ61_ARATHIPMI222

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AC004450
EMBL· GenBank· DDBJ
AAC64298.1
EMBL· GenBank· DDBJ
Genomic DNA
AC006224
EMBL· GenBank· DDBJ
AAM15163.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC10207.1
EMBL· GenBank· DDBJ
Genomic DNA
AY035158
EMBL· GenBank· DDBJ
AAK59662.1
EMBL· GenBank· DDBJ
mRNA
AY063029
EMBL· GenBank· DDBJ
AAL34203.1
EMBL· GenBank· DDBJ
mRNA
AY087084
EMBL· GenBank· DDBJ
AAM64645.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp