Q9ZVH4 · CUL3A_ARATH
- ProteinCullin-3A
- GeneCUL3A
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids732 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the cullin-RING ubiquitin ligases (CRL), or CUL3-RBX1-BTB protein E3 ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the CRL complex depends on the BTB domain-containing protein as the substrate recognition component. Involved in embryo pattern formation and endosperm development. Required for the normal division and organization of the root stem cells and columella root cap cells. Regulates primary root growth by an unknown pathway, but in an ethylene-dependent manner. Functions in distal root patterning, by an ethylene-independent mechanism. Functionally redundant with CUL3B.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | ubiquitin protein ligase binding | |
Biological Process | positive regulation of flower development | |
Biological Process | response to red or far red light | |
Biological Process | ubiquitin-dependent protein catabolic process |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameCullin-3A
- Short namesAtCUL3a
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9ZVH4
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Phenotypes & Variants
Disruption phenotype
No visible phenotype under normal growth condition. Reduced sensitivity of the inhibition of hypocotyl growth in far-red light. Cul3a and cul3b double mutant is embryonic lethal (PubMed:16045478).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 48-49 | Abolishes the interaction with AT1G21780 protein. | ||||
Sequence: SF → AA |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 14 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000396849 | 1-732 | Cullin-3A | |||
Sequence: MSNQKKRNFQIEAFKHRVVVDPKYADKTWQILERAIHQIYNQDASGLSFEELYRNAYNMVLHKFGEKLYTGFIATMTSHLKEKSKLIEAAQGGSFLEELNKKWNEHNKALEMIRDILMYMDRTYIESTKKTHVHPMGLNLWRDNVVHFTKIHTRLLNTLLDLVQKERIGEVIDRGLMRNVIKMFMDLGESVYQEDFEKPFLDASSEFYKVESQEFIESCDCGDYLKKSEKRLTEEIERVAHYLDAKSEEKITSVVEKEMIANHMQRLVHMENSGLVNMLLNDKYEDLGRMYNLFRRVTNGLVTVRDVMTSHLREMGKQLVTDPEKSKDPVEFVQRLLDERDKYDKIINTAFGNDKTFQNALNSSFEYFINLNARSPEFISLFVDDKLRKGLKGITDVDVEVILDKVMMLFRYLQEKDVFEKYYKQHLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSEDTMRGFYGSHPELSEGPTLIVQVLTTGSWPTQPAVPCNLPAEVSVLCEKFRSYYLGTHTGRRLSWQTNMGTADIKAIFGKGQKHELNVSTFQMCVLMLFNNSDRLSYKEIEQATEIPAADLKRCLQSLACVKGKNVIKKEPMSKDIGEEDLFVVNDKFTSKFYKVKIGTVVAQKETEPEKQETRQRVEEDRKPQIEAAIVRIMKSRKILDHNNIIAEVTKQLQPRFLANPTEIKKRIESLIERDFLERDSTDRKLYRYLA | ||||||
Cross-link | 676 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8) | ||||
Sequence: K |
Post-translational modification
Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed during flower development in floral meristem, sepals, petals, developing carpels, growing integuments and tetrads of megaspores. In mature ovules, expressed in the cells of the embryo sac. Expressed in the sporogenous cells, tetrads of microspores and mature pollen in stamens After fertilization, expressed in the embryo and endosperm at the globular and heart stages. The progressively decreases during ovule development and is not observed in the developing seed coat.
Gene expression databases
Interaction
Subunit
Interacts with CSN2 and RBX1A. Interacts with BTB/POZ domain-containing proteins BPM1, BPM2, BPM3, BPM6, BT1, BT2, BT3, BT5, AT1G01640, AT1G21780 and AT5G48510 (PubMed:12615944, PubMed:15618422, PubMed:15659098, PubMed:15749712, PubMed:15772280).
Interacts with SR1IP1 (PubMed:24528504).
Interacts with NPR3 and NPR4 (PubMed:22699612).
Binds to NPR1; this interaction requires NPR3 and NPR4 (PubMed:22699612, PubMed:35545668).
Interacts with SR1IP1 (PubMed:24528504).
Interacts with NPR3 and NPR4 (PubMed:22699612).
Binds to NPR1; this interaction requires NPR3 and NPR4 (PubMed:22699612, PubMed:35545668).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9ZVH4 | At1g21780 Q9XHZ8 | 4 | EBI-531362, EBI-541047 | |
BINARY | Q9ZVH4 | BPM1 Q8L765 | 5 | EBI-531362, EBI-540891 | |
BINARY | Q9ZVH4 | BPM3 O22286 | 3 | EBI-531362, EBI-540923 | |
BINARY | Q9ZVH4 | NPR1 P93002 | 3 | EBI-531362, EBI-1392127 | |
BINARY | Q9ZVH4 | RBX1A Q940X7 | 4 | EBI-531362, EBI-532404 |
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length732
- Mass (Da)85,313
- Last updated1999-05-01 v1
- Checksum40F58A46BB5572ED
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 458 | in Ref. 4; BAF02190 | ||||
Sequence: T → I |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ319540 EMBL· GenBank· DDBJ | CAC85344.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ344252 EMBL· GenBank· DDBJ | CAC87120.1 EMBL· GenBank· DDBJ | mRNA | ||
AC005508 EMBL· GenBank· DDBJ | AAD14503.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC006535 EMBL· GenBank· DDBJ | AAF87034.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE30747.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK230392 EMBL· GenBank· DDBJ | BAF02190.1 EMBL· GenBank· DDBJ | mRNA |