Q9ZU46 · ZAR1_ARATH
- ProteinReceptor protein kinase-like protein ZAR1
- GeneZAR1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids716 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Receptor protein kinase acting as an integrator for intracellular calcium and heterotrimeric G protein signaling with extracellular signals during early zygote development (PubMed:27014878).
Involved in modulating the asymmetric division of zygote and the cell fate determination of its daughter cells (PubMed:27014878).
Required for effector-triggered immunity (e.g. Pseudomonas syringae effector HopZ1a) via an activation triggered by SZE1, BKN2/SZE2 and ZED1, thus being essential for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a (PubMed:30947022).
Involved in modulating the asymmetric division of zygote and the cell fate determination of its daughter cells (PubMed:27014878).
Required for effector-triggered immunity (e.g. Pseudomonas syringae effector HopZ1a) via an activation triggered by SZE1, BKN2/SZE2 and ZED1, thus being essential for resistance against P. syringae pv. tomato DC3000 expressing HopZ1a (PubMed:30947022).
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast stroma | |
Cellular Component | plant-type cell wall | |
Cellular Component | plasma membrane | |
Cellular Component | pollen tube | |
Cellular Component | secretory vesicle | |
Molecular Function | ATP binding | |
Molecular Function | kinase activity | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity | |
Biological Process | protein autophosphorylation | |
Biological Process | zygote asymmetric cell division | |
Biological Process | zygote asymmetric cytokinesis in embryo sac |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameReceptor protein kinase-like protein ZAR1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ9ZU46
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 320-340 | Helical | ||||
Sequence: AVIAIVLCDVFGICLVGLLFT |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Embryo lethal when homozygous.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 358-373 | Loss of interaction with CAM1 or CML8. | ||||
Sequence: Missing | ||||||
Mutagenesis | 534 | Loss of kinase activity, but no effect on GB1, CAM1 or CML8 binding. | ||||
Sequence: K → A | ||||||
Mutagenesis | 577-584 | Loss of interaction with GB1. | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 60 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-20 | |||||
Sequence: MLASLIIFVALLCNVTVISG | ||||||
Chain | PRO_5006753639 | 21-716 | Receptor protein kinase-like protein ZAR1 | |||
Sequence: LNDEGFALLTFKQSVHDDPTGSLNNWNSSDENACSWNGVTCKELRVVSLSIPRKNLYGSLPSSLGFLSSLRHLNLRSNRFYGSLPIQLFHLQGLQSLVLYGNSFDGSLSEEIGKLKLLQTLDLSQNLFNGSLPLSILQCNRLKTLDVSRNNLSGPLPDGFGSAFVSLEKLDLAFNQFNGSIPSDIGNLSNLQGTADFSHNHFTGSIPPALGDLPEKVYIDLTFNNLSGPIPQTGALMNRGPTAFIGNTGLCGPPLKDLCQGYQLGLNASYPFIPSNNPPEDSDSTNSETKQKSSGLSKSAVIAIVLCDVFGICLVGLLFTYCYSKFCACNRENQFGVEKESKKRASECLCFRKDESETPSENVEHCDIVPLDAQVAFNLEELLKASAFVLGKSGIGIVYKVVLENGLTLAVRRLGEGGSQRFKEFQTEVEAIGKLKHPNIASLRAYYWSVDEKLLIYDYVSNGNLATALHGKPGMMTIAPLTWSERLRIMKGIATGLVYLHEFSPKKYVHGDLKPSNILIGQDMEPKISDFGLARLANIAGGSSPTIQSNRIIQTDQQPQERQQHHHKSVSSEFTAHSSSGSYYQAPETLKMVKPSQKWDVYSYGIILLELIAGRSPAVEVGTSEMDLVRWVQVCIEEKKPLCDVLDPCLAPEAETEDEIVAVLKIAISCVNSSPEKRPTMRHVSDTLDRLPVAGD | ||||||
Modified residue | 406 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 616 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Autophosphorylated. The kinase activity is activated by the binding of CAM1 and/or GB1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed at the micropylar nuclei of the embryo sac at eight-nucleate stage before cellularization, but not in chalazal nuclei. After cellularization, expressed in central cell and synergids in mature embryo sac. After fertilization, detected specifically in zygote, endosperm precursor cell, and later in endosperm. In addition, also present in sperm and the vegetative cells in both pollen and pollen tube. Expressed at high level in the egg cell, the central cell, the endosperm, and at low level in the zygote.
Gene expression databases
Interaction
Subunit
Part of an in vivo complex containing ZAR1, CAM1 and GB1 (PubMed:27014878).
Interacts (via CaMBD domain) with calmodulins CAM1 and CML8 in a calcium independent manner (PubMed:27014878).
Interacts (via GBeta-binding domain) with GB1 (PubMed:27014878).
Component of an immune signaling complex made of, at least, SZE1, BKN2/SZE2, ZAR1 and ZED1 (PubMed:30947022).
Binds directly to SZE1 and SZE2 at the plasma membrane (PubMed:30947022).
Interacts (via CaMBD domain) with calmodulins CAM1 and CML8 in a calcium independent manner (PubMed:27014878).
Interacts (via GBeta-binding domain) with GB1 (PubMed:27014878).
Component of an immune signaling complex made of, at least, SZE1, BKN2/SZE2, ZAR1 and ZED1 (PubMed:30947022).
Binds directly to SZE1 and SZE2 at the plasma membrane (PubMed:30947022).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q9ZU46 | At3g50230 A0A1I9LQ53 | 3 | EBI-16954860, EBI-20654045 | |
BINARY | Q9ZU46 | RLK902 Q9LVI6 | 4 | EBI-16954860, EBI-1626936 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for repeat, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 87-111 | LRR 1 | ||||
Sequence: LSSLRHLNLRSNRFYGSLPIQLFHL | ||||||
Repeat | 113-135 | LRR 2 | ||||
Sequence: GLQSLVLYGNSFDGSLSEEIGKL | ||||||
Repeat | 136-159 | LRR 3 | ||||
Sequence: KLLQTLDLSQNLFNGSLPLSILQC | ||||||
Repeat | 160-183 | LRR 4 | ||||
Sequence: NRLKTLDVSRNNLSGPLPDGFGSA | ||||||
Repeat | 185-208 | LRR 5 | ||||
Sequence: VSLEKLDLAFNQFNGSIPSDIGNL | ||||||
Repeat | 210-233 | LRR 6 | ||||
Sequence: NLQGTADFSHNHFTGSIPPALGDL | ||||||
Repeat | 234-258 | LRR 7 | ||||
Sequence: PEKVYIDLTFNNLSGPIPQTGALMN | ||||||
Region | 358-373 | CaMBD | ||||
Sequence: EKESKKRASECLCFRK | ||||||
Domain | 404-711 | Protein kinase | ||||
Sequence: KASAFVLGKSGIGIVYKVVLENGLTLAVRRLGEGGSQRFKEFQTEVEAIGKLKHPNIASLRAYYWSVDEKLLIYDYVSNGNLATALHGKPGMMTIAPLTWSERLRIMKGIATGLVYLHEFSPKKYVHGDLKPSNILIGQDMEPKISDFGLARLANIAGGSSPTIQSNRIIQTDQQPQERQQHHHKSVSSEFTAHSSSGSYYQAPETLKMVKPSQKWDVYSYGIILLELIAGRSPAVEVGTSEMDLVRWVQVCIEEKKPLCDVLDPCLAPEAETEDEIVAVLKIAISCVNSSPEKRPTMRHVSDTLDRL | ||||||
Region | 557-584 | GBeta-binding | ||||
Sequence: ANIAGGSSPTIQSNRIIQTDQQPQERQQ | ||||||
Region | 575-598 | Disordered | ||||
Sequence: TDQQPQERQQHHHKSVSSEFTAHS | ||||||
Region | 695-716 | Disordered | ||||
Sequence: PEKRPTMRHVSDTLDRLPVAGD |
Domain
The CaMBD domain (358-373) is required for calmodulin binding.
The GBeta-binding domain (577-584) is required for GB1 binding.
The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length716
- Mass (Da)78,307
- Last updated1999-05-01 v1
- Checksum80737FABB2E1C27E
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ708685 EMBL· GenBank· DDBJ | ACN59280.1 EMBL· GenBank· DDBJ | mRNA | ||
AC006200 EMBL· GenBank· DDBJ | AAD14521.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC05416.1 EMBL· GenBank· DDBJ | Genomic DNA |