Q9ZP27 · AVCO2_AVESA

  • Protein
    Avenacosidase 2
  • Gene
    P60B
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Beta-glucosidase acting as a preformed defense system. Hydrolyzes the bisdesmosides avenacosides A and B to 26-desgluco-avenacosides exhibiting fungicidal activity. Can use beta-fucoside > beta-glucoside > beta-galactoside > beta-xyloside as substrates, but not alpha-glycosides, beta-thioglucosides and disaccharides (By similarity).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.48 mMp-nitrophenyl-beta-D-glucopyranosidewith heteromultimeric recombinant enzyme
2.47 mMp-nitrophenyl-beta-D-glucopyranosidewith homodimeric recombinant enzyme

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site89a beta-D-glucoside (UniProtKB | ChEBI)
Binding site193a beta-D-glucoside (UniProtKB | ChEBI)
Binding site238-239a beta-D-glucoside (UniProtKB | ChEBI)
Active site239Proton donor
Binding site381a beta-D-glucoside (UniProtKB | ChEBI)
Active site454Nucleophile
Binding site454a beta-D-glucoside (UniProtKB | ChEBI)
Binding site504a beta-D-glucoside (UniProtKB | ChEBI)
Binding site511-512a beta-D-glucoside (UniProtKB | ChEBI)
Binding site520a beta-D-glucoside (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast stroma
Molecular Functionbeta-glucosidase activity
Biological Processcarbohydrate metabolic process
Biological Processdefense response

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GH1Glycoside Hydrolase Family 1

Names & Taxonomy

Protein names

  • Recommended name
    Avenacosidase 2
  • EC number
  • Alternative names
    • 26-desgluco-avenacosidase 2
    • Protein As-Glu2

Gene names

    • Name
      P60B
    • Synonyms
      GLU2

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Garry
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Pooideae > Poodae > Poeae > Poeae Chloroplast Group 1 (Aveneae type) > Aveninae > Avena

Accessions

  • Primary accession
    Q9ZP27

Subcellular Location

Note: Found in a fibrillar spherulite called stromacentre.

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain, disulfide bond.

TypeIDPosition(s)Description
Transit peptide1-57Chloroplast
ChainPRO_000042864158-578Avenacosidase 2
Disulfide bond258↔264

Keywords

Interaction

Subunit

Heteromultimer with P60A in a 1:1 stoichiometry. Aggregates to form the fibrillar stromacentre.

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    578
  • Mass (Da)
    65,693
  • Last updated
    1999-05-01 v1
  • Checksum
    FA800D307BAEB7E8
MALLCSALSNSTHPSFRSHIAGANSENLWHLSAHPAQKSKRRCNLTLSSRAAARISSALESGKLKPWQIPKRDWFPPEFTFGAASAAYQIEGAWNEGGKGPSSWDNFCHNYPERIMDGSNWDVAANSYYMYKEDVRMLKEIGMDSYRFSISWPRILPEGTLEGGINHEGIQYYNDLLDCLIENGIKPYITLFHWDTPQALADKYNDFLDRRIVKDYTDYATVCFEHFGDKVKNWITFNEPHSFCGLAYGTGLHAPGLCSPGMDCAIPQGDALRQPYIVGHNLLLAHAETVDVYKKFYKGDDGQIGMVMDVMAYEPYGNNFVDQQAQERSIDFHIGWFLEPMVRGDYPFSMRSLVGDRLPFFTKSEQEKLVSSYDFVGINYYTARFSEHIDISPEIIPKLNTDDAYSTPEFNDSNGIPIGPDLGMYWILSYPKGLKDILLLMKEKYGNPPIYITENGTADMDGWGNPPMTDPLDDPLRIEYLQQHMTAIKEAIDLGADVRGHFTWSLIDNFEWSMGYLSRFGIVYIDRNDGFKRIMKKSAKWLKEFNGATKEVNNKILGASSCCSGELMWFLVQNPYGK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF082991
EMBL· GenBank· DDBJ
AAD02839.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

Disclaimer

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