Q9ZCE9 · PBPA_RICPR
- ProteinPenicillin-binding protein 1A
- GenemrcA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids787 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
Catalytic activity
- [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H+
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate RHEA-COMP:9602 + CHEBI:60033 = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate RHEA-COMP:9603 + CHEBI:58405 + CHEBI:15378
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 87 | Proton donor; for transglycosylase activity | ||||
Sequence: E | ||||||
Active site | 457 | Acyl-ester intermediate; for transpeptidase activity | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | penicillin binding | |
Molecular Function | peptidoglycan glycosyltransferase activity | |
Molecular Function | serine-type D-Ala-D-Ala carboxypeptidase activity | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | proteolysis | |
Biological Process | regulation of cell shape | |
Biological Process | response to antibiotic |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePenicillin-binding protein 1A
- Short namesPBP-1a; PBP1a
Including 2 domains:
- Recommended namePenicillin-insensitive transglycosylase
- EC number
- Alternative names
- Recommended namePenicillin-sensitive transpeptidase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rickettsiales > Rickettsiaceae > Rickettsieae > Rickettsia > typhus group
Accessions
- Primary accessionQ9ZCE9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-6 | Cytoplasmic | ||||
Sequence: MYKSLF | ||||||
Transmembrane | 7-27 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: LCLKIFAVLILIGCSVTAYII | ||||||
Topological domain | 28-787 | Periplasmic | ||||
Sequence: YHYSHDLPDYSQLARYYPPSVTRIYSRDGKLIEEYAFERRVFVPINNVPSSLIESFIAAEDKNFYTHPGIDLLGIVRAAFLNISNYLHNRRMEGASTITQQVVKNFLLTNEVSLERKIKEVIISYMISRIFTKHQILELYLNQTFFGRGAYGVAAAAQNYFNKSVEELTIAESAFIAALPKAPSELNPDKNYSRVKARRDYVIERMFEDGYITRDTMKEAIGSPIVLRKRAKEETVTADYYAEQVREEVIRMLNSKEEFYRGGLTIITSLDAKMQQLAENSLRKGLREFDRKSGFRKPIANIPLDNWQEELKKLPTPSSLLEYKLAVVLDVSDNHAKIGLIDGSKAKIPIVEMQWARSNLKSVKTLLKKGDVIVVEPIKDCYALRQIPEVNGAIMVMNPHTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFNDGPIEIIQGPGMPSWCPKNYEGQFLGDMTMRTGFEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETTLSRITNAYAIIANGGKKVEPHFVELIQDRNGKIIYRRDNRECFSCNIADSDLDTAILEIPKEDIYRVTDEASNYQITSFLTGAIDSGTGYAARKLGKIIAGKTGTSNDSKDTWFIGFTPKIVVGSYVGYDTPKELGKKATGSNVVLPIFIDFMNHAYKDEPSLPFKVPDSIKLIAVDRITGKMIPNGTVIEAFKVNNIQMLENDYMIDNHDIFDYVPGMLDQSQEIY |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000083174 | 1-787 | Penicillin-binding protein 1A | |||
Sequence: MYKSLFLCLKIFAVLILIGCSVTAYIIYHYSHDLPDYSQLARYYPPSVTRIYSRDGKLIEEYAFERRVFVPINNVPSSLIESFIAAEDKNFYTHPGIDLLGIVRAAFLNISNYLHNRRMEGASTITQQVVKNFLLTNEVSLERKIKEVIISYMISRIFTKHQILELYLNQTFFGRGAYGVAAAAQNYFNKSVEELTIAESAFIAALPKAPSELNPDKNYSRVKARRDYVIERMFEDGYITRDTMKEAIGSPIVLRKRAKEETVTADYYAEQVREEVIRMLNSKEEFYRGGLTIITSLDAKMQQLAENSLRKGLREFDRKSGFRKPIANIPLDNWQEELKKLPTPSSLLEYKLAVVLDVSDNHAKIGLIDGSKAKIPIVEMQWARSNLKSVKTLLKKGDVIVVEPIKDCYALRQIPEVNGAIMVMNPHTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFNDGPIEIIQGPGMPSWCPKNYEGQFLGDMTMRTGFEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETTLSRITNAYAIIANGGKKVEPHFVELIQDRNGKIIYRRDNRECFSCNIADSDLDTAILEIPKEDIYRVTDEASNYQITSFLTGAIDSGTGYAARKLGKIIAGKTGTSNDSKDTWFIGFTPKIVVGSYVGYDTPKELGKKATGSNVVLPIFIDFMNHAYKDEPSLPFKVPDSIKLIAVDRITGKMIPNGTVIEAFKVNNIQMLENDYMIDNHDIFDYVPGMLDQSQEIY |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 49-220 | Transglycosylase | ||||
Sequence: TRIYSRDGKLIEEYAFERRVFVPINNVPSSLIESFIAAEDKNFYTHPGIDLLGIVRAAFLNISNYLHNRRMEGASTITQQVVKNFLLTNEVSLERKIKEVIISYMISRIFTKHQILELYLNQTFFGRGAYGVAAAAQNYFNKSVEELTIAESAFIAALPKAPSELNPDKNYS | ||||||
Region | 398-711 | Transpeptidase | ||||
Sequence: DVIVVEPIKDCYALRQIPEVNGAIMVMNPHTGQVLASVGGYDFSTSKFDRVTQALRQPGSLSKTFVYLAALENGVKPNQIFNDGPIEIIQGPGMPSWCPKNYEGQFLGDMTMRTGFEKSRNLITVRVATAVGLTKIVDIIKRFGINNEPKKVYSMVLGSIETTLSRITNAYAIIANGGKKVEPHFVELIQDRNGKIIYRRDNRECFSCNIADSDLDTAILEIPKEDIYRVTDEASNYQITSFLTGAIDSGTGYAARKLGKIIAGKTGTSNDSKDTWFIGFTPKIVVGSYVGYDTPKELGKKATGSNVVLPIFID |
Sequence similarities
In the N-terminal section; belongs to the glycosyltransferase 51 family.
In the C-terminal section; belongs to the transpeptidase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length787
- Mass (Da)88,694
- Last updated1999-05-01 v1
- ChecksumE9BF4429D17A32D0
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ235273 EMBL· GenBank· DDBJ | CAA15233.1 EMBL· GenBank· DDBJ | Genomic DNA |