Q9Z6M7 · AAXB_CHLPN
- ProteinPyruvoyl-dependent arginine decarboxylase AaxB
- GeneaaxB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids195 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Part of the AaxABC system, catalyzes the decarboxylation of L-arginine. The arginine uptake by the bacterium in the macrophage may be a virulence factor against the host innate immune response.
Catalytic activity
- H+ + L-arginine = agmatine + CO2
Cofactor
Note: Binds 1 pyruvoyl group covalently per subunit.
Activity regulation
Inhibited by argininamide.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
5 mM | L-arginine |
pH Dependence
Optimum pH is 3.4.
Temperature Dependence
Thermostable. Retains 48% activity at 50 degrees Celsius, and 13% activity at 100 degrees Celsius.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 52-53 | Cleavage (non-hydrolytic) | ||||
Sequence: TS |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | arginine decarboxylase activity | |
Biological Process | arginine catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvoyl-dependent arginine decarboxylase AaxB
- EC number
- Short namesPvlArgDC
- Alternative names
- Cleaved into 2 chains
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Chlamydiota > Chlamydiia > Chlamydiales > Chlamydiaceae > Chlamydia/Chlamydophila group > Chlamydia
Accessions
- Primary accessionQ9Z6M7
- Secondary accessions
Proteomes
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 52 | 70% loss of activity. Significantly impaired in ability to self-cleave. | ||||
Sequence: T → S |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000364033 | 1-52 | Pyruvoyl-dependent arginine decarboxylase subunit beta | |||
Sequence: MAYGTRYPTLAFHTGGIGESDDGMPPQPFETFCYDSALLQAKIENFNIVPYT | ||||||
Modified residue | 53 | Pyruvic acid (Ser) | ||||
Sequence: S | ||||||
Chain | PRO_0000364034 | 53-195 | Pyruvoyl-dependent arginine decarboxylase subunit alpha | |||
Sequence: SVLPKELFGNIVPVDTCVKSFKHGAVLEVIMAGRGAALSDGTHAIATGIGICWGKDKNGELIGGWAAEYVEFFPTWINDEIAETHAKMWLKKSLQHELDLRSIAKHSEFQFFHNYINIKQKFGFCLTALGFLNFENAEPAKVN |
Interaction
Structure
Sequence
- Sequence statusComplete
- Length195
- Mass (Da)21,655
- Last updated1999-05-01 v1
- Checksum1506883FB4854DA2
Mass Spectrometry
Pyruvoyl-dependent arginine decarboxylase subunit alpha
Molecular mass is 15,875 Da. Determined by MALDI.Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE001363 EMBL· GenBank· DDBJ | AAD19169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE002161 EMBL· GenBank· DDBJ | AAF38615.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000008 EMBL· GenBank· DDBJ | BAA99239.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE009440 EMBL· GenBank· DDBJ | AAP99001.1 EMBL· GenBank· DDBJ | Genomic DNA |