Q9Z2J0 · S23A1_MOUSE
- ProteinSolute carrier family 23 member 1
- GeneSlc23a1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids605 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sodium:L-ascorbate cotransporter. Mediates electrogenic uptake of vitamin C, with a stoichiometry of 2 Na+ for each L-ascorbate (PubMed:36749388).
Has retained some ancestral activity toward nucleobases such as urate, an oxidized purine. Low-affinity high-capacity sodium:urate cotransporter, may regulate serum urate levels by serving as a renal urate re-absorber (By similarity) (PubMed:36749388).
Has retained some ancestral activity toward nucleobases such as urate, an oxidized purine. Low-affinity high-capacity sodium:urate cotransporter, may regulate serum urate levels by serving as a renal urate re-absorber (By similarity) (PubMed:36749388).
Catalytic activity
- L-ascorbate(out) + 2 Na+(out) = L-ascorbate(in) + 2 Na+(in)This reaction proceeds in the forward direction.L-ascorbate (out)CHEBI:38290
+ 2 Na+ (out)CHEBI:29101= L-ascorbate (in)CHEBI:38290+ 2 Na+ (in)CHEBI:29101 - urate(out) + 2 Na+(out) = urate(in) + 2 Na+(in)This reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
646 μM | urate |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
430.1 pmol/min/mg | toward urate |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basal plasma membrane | |
Cellular Component | cytoplasm | |
Cellular Component | intracellular organelle | |
Cellular Component | plasma membrane | |
Molecular Function | dehydroascorbic acid transmembrane transporter activity | |
Molecular Function | L-ascorbate:sodium symporter activity | |
Molecular Function | L-ascorbic acid transmembrane transporter activity | |
Molecular Function | sodium ion transmembrane transporter activity | |
Molecular Function | urate transmembrane transporter activity | |
Biological Process | brain development | |
Biological Process | dehydroascorbic acid transport | |
Biological Process | L-ascorbic acid transmembrane transport | |
Biological Process | lung development | |
Biological Process | response to toxic substance | |
Biological Process | sodium ion transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSolute carrier family 23 member 1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ9Z2J0
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-59 | Cytoplasmic | ||||
Sequence: MKTPEDPGSPKQHEVVDSAGTSTRDRQAPLPTEPKFDMLYKIEDVPPWYLCILLGFQHY | ||||||
Transmembrane | 60-80 | Helical | ||||
Sequence: LTCFSGTIAVPFLLAEALCVG | ||||||
Topological domain | 81-88 | Extracellular | ||||
Sequence: RDQHMVSQ | ||||||
Transmembrane | 89-109 | Helical | ||||
Sequence: LIGTIFTCVGITTLIQTTVGI | ||||||
Topological domain | 110 | Cytoplasmic | ||||
Sequence: R | ||||||
Transmembrane | 111-131 | Helical | ||||
Sequence: LPLFQASAFAFLVPAKSILAL | ||||||
Topological domain | 132-166 | Extracellular | ||||
Sequence: ERWKCPSEEEIYGNWSMPLNTSHIWHPRIREVQGA | ||||||
Transmembrane | 167-187 | Helical | ||||
Sequence: IMVSSMVEVVIGLMGLPGALL | ||||||
Topological domain | 188-214 | Cytoplasmic | ||||
Sequence: SYIGPLTVTPTVSLIGLSVFQAAGDRA | ||||||
Transmembrane | 215-232 | Helical | ||||
Sequence: GSHWGISACSILLIVLFS | ||||||
Topological domain | 233-236 | Extracellular | ||||
Sequence: QYLR | ||||||
Intramembrane | 237-250 | Helical | ||||
Sequence: NLTFLLPVYRWGKG | ||||||
Topological domain | 251-257 | Extracellular | ||||
Sequence: LTLFRVQ | ||||||
Transmembrane | 258-278 | Helical | ||||
Sequence: IFKMFPIVLAIMTVWLLCYVL | ||||||
Topological domain | 279-319 | Cytoplasmic | ||||
Sequence: TLTDVLPADPTVYGFQARTDARGDIMAISPWIRIPYPCQWG | ||||||
Transmembrane | 320-340 | Helical | ||||
Sequence: LPTVTVAAVLGMFSATLAGII | ||||||
Topological domain | 341-365 | Extracellular | ||||
Sequence: ESIGDYYACARLAGAPPPPVHAINR | ||||||
Transmembrane | 366-386 | Helical | ||||
Sequence: GIFTEGICCIIAGLLGTGNGS | ||||||
Topological domain | 387-409 | Cytoplasmic | ||||
Sequence: TSSSPNIGVLGITKVGSRRVVQY | ||||||
Transmembrane | 410-430 | Helical | ||||
Sequence: GAGIMLILGAIGKFTALFASL | ||||||
Topological domain | 431-433 | Extracellular | ||||
Sequence: PDP | ||||||
Transmembrane | 434-454 | Helical | ||||
Sequence: ILGGMFCTLFGMITAVGLSNL | ||||||
Topological domain | 455-464 | Cytoplasmic | ||||
Sequence: QFVDMNSSRN | ||||||
Transmembrane | 465-485 | Helical | ||||
Sequence: LFVLGFSMFFGLTLPNYLDSN | ||||||
Topological domain | 486-497 | Extracellular | ||||
Sequence: PGAINTGIPEVD | ||||||
Transmembrane | 498-518 | Helical | ||||
Sequence: QILTVLLTTEMFVGGCLAFIL | ||||||
Topological domain | 519-605 | Cytoplasmic | ||||
Sequence: DNTVPGSPEERGLIQWKAGAHANSETSASLKSYDFPFGMGMVKRTTFFRYIPICPVFRGFSKKTQNQPPVLEDTPDNIETGSVCTKV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000165976 | 1-605 | Solute carrier family 23 member 1 | |||
Sequence: MKTPEDPGSPKQHEVVDSAGTSTRDRQAPLPTEPKFDMLYKIEDVPPWYLCILLGFQHYLTCFSGTIAVPFLLAEALCVGRDQHMVSQLIGTIFTCVGITTLIQTTVGIRLPLFQASAFAFLVPAKSILALERWKCPSEEEIYGNWSMPLNTSHIWHPRIREVQGAIMVSSMVEVVIGLMGLPGALLSYIGPLTVTPTVSLIGLSVFQAAGDRAGSHWGISACSILLIVLFSQYLRNLTFLLPVYRWGKGLTLFRVQIFKMFPIVLAIMTVWLLCYVLTLTDVLPADPTVYGFQARTDARGDIMAISPWIRIPYPCQWGLPTVTVAAVLGMFSATLAGIIESIGDYYACARLAGAPPPPVHAINRGIFTEGICCIIAGLLGTGNGSTSSSPNIGVLGITKVGSRRVVQYGAGIMLILGAIGKFTALFASLPDPILGGMFCTLFGMITAVGLSNLQFVDMNSSRNLFVLGFSMFFGLTLPNYLDSNPGAINTGIPEVDQILTVLLTTEMFVGGCLAFILDNTVPGSPEERGLIQWKAGAHANSETSASLKSYDFPFGMGMVKRTTFFRYIPICPVFRGFSKKTQNQPPVLEDTPDNIETGSVCTKV | ||||||
Glycosylation | 145 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 151 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 598 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 600 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 603 | Phosphothreonine | ||||
Sequence: T |
Post-translational modification
Phosphorylated.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in kidney (at protein level).
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-30 | Disordered | ||||
Sequence: MKTPEDPGSPKQHEVVDSAGTSTRDRQAPL |
Sequence similarities
Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length605
- Mass (Da)65,554
- Last updated2002-10-19 v2
- ChecksumA3F64CB42DA1CFF5
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A494BAL1 | A0A494BAL1_MOUSE | Slc23a1 | 53 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 205 | in Ref. 1; AAC78805 | ||||
Sequence: S → Y | ||||||
Sequence conflict | 223 | in Ref. 2; BAC39457 | ||||
Sequence: C → R | ||||||
Sequence conflict | 540-541 | in Ref. 2; BAC39457 | ||||
Sequence: AN → PH | ||||||
Sequence conflict | 579 | in Ref. 2; BAC39457 | ||||
Sequence: S → F |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF058318 EMBL· GenBank· DDBJ | AAC78805.1 EMBL· GenBank· DDBJ | mRNA | ||
AK085499 EMBL· GenBank· DDBJ | BAC39457.1 EMBL· GenBank· DDBJ | mRNA | ||
AK143959 EMBL· GenBank· DDBJ | BAE25631.1 EMBL· GenBank· DDBJ | mRNA | ||
AK165439 EMBL· GenBank· DDBJ | BAE38187.1 EMBL· GenBank· DDBJ | mRNA | ||
BC013528 EMBL· GenBank· DDBJ | AAH13528.1 EMBL· GenBank· DDBJ | mRNA |